{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:35:31Z","timestamp":1790354131160,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T00:00:00Z","timestamp":1588896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Office of Research and Sponsored Programs at the University of Toledo"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Potent inhibitors of an essential microbial enzyme have been shown to be effective growth inhibitors of <jats:italic>Candida albicans<\/jats:italic>, a pathogenic fungus. <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> is the main cause of oropharyngeal candidiasis, and also causes invasive fungal infections, including systemic sepsis, leading to serious complications in immunocompromised patients. As the rates of drug\u2010resistant fungal infections continue to rise novel antifungal treatments are desperately needed. The enzyme aspartate semialdehyde dehydrogenase (ASADH) is critical for the functioning of the aspartate biosynthetic pathway in microbes and plants. Because the aspartate pathway is absent in humans, ASADH has the potential to be a promising new target for antifungal research. Deleting the <jats:italic>asd<\/jats:italic> gene encoding for ASADH significantly decreases the survival of <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic>, establishing this enzyme as essential for this organism. Previously developed ASADH inhibitors were tested against several strains of <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> to measure their possible therapeutic impact. The more potent inhibitors show a good correlation between enzyme inhibitor potency and fungal growth inhibition. Growth curves generated by incubating different <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> strains with varying enzyme inhibitor levels show significant slowing of fungal growth by these inhibitors against each of these strains, similar to the effect observed with a clinical antifungal drug. The most effective inhibitors also demonstrated relatively low cytotoxicity against a human epithelial cell line. Taken together, these results establish that the ASADH enzyme is a promising new target for further development as a novel antifungal treatment against <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> and related fungal species.<\/jats:p>","DOI":"10.1002\/ddr.21682","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T10:11:55Z","timestamp":1588932715000},"page":"736-744","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Aspartate semialdehyde dehydrogenase inhibition suppresses the growth of the pathogenic fungus <scp><i>Candida albicans<\/i><\/scp>"],"prefix":"10.1002","volume":"81","author":[{"given":"Gopal P.","family":"Dahal","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Toledo  Toledo Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dylan","family":"Launder","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Toledo  Toledo Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine M. M.","family":"McKeone","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Toledo  Toledo Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph P.","family":"Hunter","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Toledo  Toledo Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather R.","family":"Conti","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Toledo  Toledo Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3117-9168","authenticated-orcid":false,"given":"Ronald E.","family":"Viola","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Toledo  Toledo Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,8]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Reference methods for broth dilution antifungal susceptibility testing of yeasts","author":"Alexander B. 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