{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T01:07:41Z","timestamp":1782868061233,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T00:00:00Z","timestamp":1601596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sinomenine (SN) is a plant\u2010derived alkaloid isolated from Caulis Sinomenii. It has been approved by the State Food and Drug Administration of China for treating rheumatoid arthritis (RA) nearly 20\u2009years ago. To investigate the anti\u2010RA mechanism of SN, a lot of scholars reported the immunosuppressive effect of SN on T lymphocytes. We continued to evaluate the suppressive function of SN by using human peripheral blood mononuclear cells (PBMCs) isolated from RA patients. As the positive control, 10 ng\/ml of methylprednisolone (MP) showed the antiproliferation effect on mitogen\u2010activated PBMCs of RA patients significantly (*<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Meanwhile, MP decreased the frequency of CD4<jats:sup>+<\/jats:sup> CD25<jats:sup>+<\/jats:sup> T cells and suppressed the secretion of inflammatory Th1\/Th2\/Th17 cytokines such as IL\u20104, IL\u20106, IL\u201010, IL\u201017, IFN\u2010\u03b3, and TNF\u2010\u03b1. However, SN at concentrations of 0.3\u201330\u2009\u03bcM, showed little suppressive effects on the proliferation of PBMCs of RA patients. We did not observe any suppressive effects of SN on percentages of CD4<jats:sup>+<\/jats:sup> T cells and CD4<jats:sup>+<\/jats:sup> CD25<jats:sup>+<\/jats:sup> T cells in the mitogen\u2010activated PBMCs of RA patients. The influence of SN on the percentage of CD4<jats:sup>+<\/jats:sup> CD25<jats:sup>+<\/jats:sup> Foxp3<jats:sup>+<\/jats:sup> T cells was also limited. Finally, even 30\u2009\u03bcM of SN did not influence the secretion of Th1\/Th2\/Th17 cytokine significantly. The present study provided evidence that anti\u2010RA mechanism of SN seems not to be related with the suppressive effects on peripheral T cells.<\/jats:p>","DOI":"10.1002\/ddr.21748","type":"journal-article","created":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T05:15:10Z","timestamp":1601615710000},"page":"251-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Effects of sinomenine on the proliferation, cytokine production, and regulatory T\u2010cell frequency in peripheral blood mononuclear cells of rheumatoid arthritis patients"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4065-1548","authenticated-orcid":false,"given":"Wencheng","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Pharmacy Hubei Provincial Hospital of Traditional Chinese Medicine  Wuhan China"},{"name":"Institute of Traditional Chinese Medicine Hubei Province Academy of 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