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Whether the expression of HDAC6 and its new inhibitor ACY1215 can inhibit the proliferation of gallbladder cancer cells and induce their apoptosis remains to be further studied. The purpose of this study was to explore the effects of ACY1215 on the gallbladder cancer cells. Cell proliferation of GBC\u2010SD and SGC\u2010996 was assessed by cell counting kit\u20108 assay and colony formation assay. Flow cytometry was used to detect the apoptosis of gallbladder cancer cells. Western blot was used to detect the expressions of PCNA\uff0cKI67, and apoptosis\u2010related proteins of gallbladder cancer cells. The HDAC6 inhibitor ACY1215 suppressed the proliferation of GBC\u2010SD and SDC\u2010996 cells and promoted the apoptosis of gallbladder cancer cells. The HDAC6 inhibitor ACY1215 increases the chemotherapy effect of gemcitabine and oxaliplatin. ACY1215 could suppress cell proliferation and induce apoptosis of GBC\u2010SD and SGC\u2010996, and increased the chemotherapy effect of gemcitabine and oxaliplatin, which provides a rationale for the combination of HDAC6 selective inhibitors with other anticancer agents in treating gallbladder cancer.<\/jats:p>","DOI":"10.1002\/ddr.21780","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T01:21:33Z","timestamp":1610673693000},"page":"598-604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["<scp>HDAC6<\/scp> inhibitor, <scp>ACY1215<\/scp> suppress the proliferation and induce apoptosis of gallbladder cancer cells and increased the chemotherapy effect of gemcitabine and oxaliplatin"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4303-1953","authenticated-orcid":false,"given":"Yi","family":"Ruan","sequence":"first","affiliation":[{"name":"Department of Minimal Invasive Surgery Ninbo Medical Center Lihuili Hospital  Ningbo Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luoluo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Minimal Invasive Surgery Ninbo Medical Center Lihuili Hospital  Ningbo Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeting","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Minimal Invasive Surgery Ninbo Medical Center Lihuili Hospital  Ningbo Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/phar.1671"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2009-0188"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2012.06.014"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2011.07048.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5167"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms14059514"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/iep.12132"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2019.03.003"},{"key":"e_1_2_8_10_1","first-page":"99","article-title":"Gallbladder cancer: Epidemiology and outcome","volume":"6","author":"Hundal R.","year":"2014","journal-title":"Clinical Epidemiology"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0290175"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4143\/crt.2013.45.2.134"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-007-0085-0"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2020.106398"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.04.021"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2015.08.007"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm401899x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/13543776.2018.1508451"},{"issue":"6","key":"e_1_2_8_19_1","first-page":"1509","article-title":"Effects of the HDAC inhibitor CG2 in combination with irinotecan, 5\u2010fluorouracil, or oxaliplatin on HCT116 colon cancer cells and xenografts","volume":"24","author":"Na Y. 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