{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:04:38Z","timestamp":1787241878466,"version":"build-2736575974"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T00:00:00Z","timestamp":1625529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Periodontitis is a serious global concern. Therefore, in the present study, we intend to synthesize novel valproic\u2010acid pyrazole conjugates as a novel agent against periodontitis. The molecules were developed in a facile synthetic route and obtained in excellent yields. The entire set of molecules were screened for antibacterial activity against a battery of micro\u2010organisms responsible for periodontitis such as\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    ,\n                    <jats:italic>P. intermedia<\/jats:italic>\n                    ,\n                    <jats:italic>F. nucleatum,<\/jats:italic>\n                    and\n                    <jats:italic>E. coli<\/jats:italic>\n                    , where they exhibit considerable inhibitory activity. The most potent compound among the tested series, compound\n                    <jats:bold>7c<\/jats:bold>\n                    showed bactericidal activity in the time\u2010kill curve against\n                    <jats:italic>E. coli<\/jats:italic>\n                    . Compound\n                    <jats:bold>7c<\/jats:bold>\n                    also showed inhibition of NF\u2010\u0138B transcriptional activity in LPS\u2010stimulated RAW264.7 cells with IC\n                    <jats:sub>50<\/jats:sub>\n                    of 19.23\u2009\u03bcM. The effect of compound\n                    <jats:bold>7c<\/jats:bold>\n                    was also investigated in experimentally induced periodontitis in rats on various indices of oxidative stress (MDA, SOD, and GSH), inflammation (TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u20106), and apoptosis. It has been found that compound\n                    <jats:bold>7c<\/jats:bold>\n                    significantly inhibits oxidative stress, inflammation, and apoptosis in a dose\u2010dependent manner. Compound\n                    <jats:bold>7c<\/jats:bold>\n                    also inhibits the expression of COX\u20102 and iNOS as shown by western blot analysis.\n                  <\/jats:p>","DOI":"10.1002\/ddr.21851","type":"journal-article","created":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T03:39:00Z","timestamp":1625542740000},"page":"131-141","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Antibacterial and anti\u2010inflammatory activity of valproic acid\u2010pyrazole conjugates as a potential agent against periodontitis"],"prefix":"10.1002","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7099-8943","authenticated-orcid":false,"given":"Lei","family":"Dong","sequence":"first","affiliation":[{"name":"School of Stomatology Anhui Medical College  Heifei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Stomatology Huangshan City People's Hospital Huangshan  Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxiang","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Stomatology The Affiliated Anqing Hospital of Anhui Medical University  Anqing Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Stomatology Anhui Medical College  Heifei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Stomatology Anhui Medical College  Heifei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Stomatology Anhui Medical College  Heifei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6NJ03181A"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/479364"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJMR2017.8545"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3785"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23010134"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2016.04.077"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.4.547-558.2000"},{"key":"e_1_2_7_10_1","volume-title":"Synthesis and characterization of pharmacologically active compounds","author":"Parekh S. 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