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This study explored the mechanism of Calycosin in MSU\u2010induced gouty arthritis. MSU\u2010induced gouty arthritis mouse models with or without treatment of Calycosin were established, and physiological and pathological indicators were determined. Similarly, peripheral blood mononuclear cells (PBMCs) and THP\u20101 macrophages were used in vitro. Lactate dehydrogenase (LDH) was tested. The degree of centrifugal infiltration was detected by immunofluorescence. ELISA and quantitative reverse\u2010transcription polymerase chain reaction\u00a0were conducted to determine the levels of inflammatory factors. Immunohistochemistry, immunofluorescence, and flow cytometry were utilized to detect the content of caspase\u20101. Protein expressions of NF\u2010\u03baB\u2010, p62\u2010Keap1 pathway\u2010, and pyroptosis\u2010related factors were examined by western blot. In MSU\u2010induced mouse models, calycosin increased mechanical hyperalgesia but decreased the swelling index of the mouse knee joint in a time\u2010dependent manner. MSU treatment increased inflammatory cells and LysM\u2010eGFP<jats:sup>+<\/jats:sup> neutrophils recruitment in vivo, and promoted the LDH content in vitro,\u00a0and meanwhile, calycosin reversed the aforementioned effects of MSU. In addition, calycosin repressed the release of inflammatory factors, promoted p62 level and diminished the levels of AIM2, caspase\u20101, ASC, IL\u20101\u03b2, Keap1, Cleaved GSDMD, and Cleaved caspase\u20101 and phosphorylation of p65 and I\u03baB\u03b1 in MSU\u2010induced mouse or cell models. Furthermore, AIM2 silencing also inhibited MSU\u2010induced inflammation and pyroptosis. Collectively, calycosin may inhibit AIM2 inflammasomes\u2010mediated inflammation and pyroptosis through NF\u2010\u03baB and p62\u2010Keap1 pathways, ultimately playing a protective role in gouty arthritis.<\/jats:p>","DOI":"10.1002\/ddr.21985","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T07:04:36Z","timestamp":1662534276000},"page":"1654-1672","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Calycosin represses AIM2 inflammasome\u2010mediated inflammation and pyroptosis to attenuate monosodium urate\u2010induced gouty arthritis through NF\u2010\u03baB and p62\u2010Keap1 pathways"],"prefix":"10.1002","volume":"83","author":[{"given":"Jing","family":"Tian","sequence":"first","affiliation":[{"name":"Department of Orthopaedics General Hospital of Northern Theater Command  Shenyang 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