{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T03:03:47Z","timestamp":1789095827619,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702648"],"award-info":[{"award-number":["81702648"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772850"],"award-info":[{"award-number":["81772850"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Artemether (ATM) is a natural antimalarial drug that can also regulate glucose and lipid metabolism. However, little is known regarding its pharmacological action in metabolic dysfunction\u2010associated fatty liver disease (MAFLD), and the underlying mechanisms remain undetermined. The aim of this study was to explore the therapeutic effects of ATM against hepatic steatosis and the possible mechanisms. ATM significantly decreased blood glucose levels, improved glucose tolerance, reduced inflammatory response, and alleviated hepatic steatosis in the ob\/ob mouse model as well as the high\u2010fat diet\u2010fed mice. ATM also inhibited lipid accumulation in murine hepatocytes in vitro. Using RNA sequencing, miR\u201034a\u20105p and peroxisome proliferator\u2010activated receptor\u2010\u03b1\u00a0(PPAR\u03b1) were identified as important regulators during ATM treatment. ATM administration downregulated miR\u201034a\u20105p expression and miR\u201034a\u20105p abrogated the inhibitory effects of ATM on PO (palmitate\u2009+\u2009oleate)\u2010induced lipid accumulation as well as triglycerides levels in murine hepatocytes. Furthermore, the expression of PPAR\u03b1, a target gene of miR\u201034a\u20105p, was upregulated by ATM and PPAR\u03b1 inhibitor MK\u2010886 abolished the positive effect of ATM. Consequently, PPAR\u03b1 agonist fenofibrate reversed the decreased mitochondrial fatty acid \u03b2\u2010oxidation induced by miR\u201034a\u20105p mimics after ATM treatment, thereby leading to attenuation of intracellular lipid accumulation. Taken together, ATM is a promising therapeutic agent against MAFLD that reduces lipid deposition by suppressing miR\u201034a\u20105p and upregulating PPAR\u03b1.<\/jats:p>","DOI":"10.1002\/ddr.22020","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T05:00:15Z","timestamp":1671598815000},"page":"156-171","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Therapeutic role of Artemether in the prevention of\u00a0hepatic\u00a0steatosis through miR\u201034a\u20105p\/PPAR\u03b1 pathway"],"prefix":"10.1002","volume":"84","author":[{"given":"Li","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The First Affiliated 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