{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T01:05:11Z","timestamp":1760403911720,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T00:00:00Z","timestamp":1683763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Facing the sudden outbreak of coronavirus disease 2019 (COVID\u201019), it is extremely urgent to develop effective antiviral drugs against severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102). Drug repurposing is a promising strategy for the treatment of COVID\u201019. To identify the precise target protein of marketed medicines, we initiate a chemical biological program to identify precise target of potential antivirus drugs. In this study, two types of recombinant human coronavirus SARS\u2010CoV\u20102 RdRp protein capturing probes with various photoaffinity labeling units were designed and synthesized based on the structure of FDA\u2010approved drugs stavudine, remdesivir, acyclovir, and aladenosine. Fortunately, it was found that one novel photoaffinity probe, RD\u20101, could diaplayed good affinity with SARS\u2010CoV\u20102 RdRp around the residue ARG_553. In addition, RD\u20101 probe also exhibited potent inhibitory activity against 3CL<jats:sup>pro<\/jats:sup> protease. Taken together, our findings will elucidate the structural basis for the efficacy of marketed drugs, and explore a rapid and efficient strategy of drug repurposing based on the identification of new targets. Moreover, these results could also provide a scientific basis for the clinical application of marketed drugs.<\/jats:p>","DOI":"10.1002\/ddr.22075","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T04:14:12Z","timestamp":1683778452000},"page":"1142-1158","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of potential targets against SARS\u2010CoV\u20102 of antiviral drugs based on photoaffinity probes"],"prefix":"10.1002","volume":"84","author":[{"given":"Yuexiang","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Xi'an Jiaotong University Xi'an China"},{"name":"Department of Emergency, Xijing Hospital Air Force Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Health Science Center Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Pan","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Health Science Center Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2727-8806","authenticated-orcid":false,"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Health Science Center Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.153165"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4546-4554.2005"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26113461"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.13403"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15372"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202102036"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb7498"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1RA04831G"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/2040206618764483"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2021.1894985"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmedchemlett.5b00318"},{"issue":"4","key":"e_1_2_8_13_1","article-title":"Impact of chronic kidney disease on severity and mortality in COVID\u201019 patients: A systematic review and meta\u2010analysis","volume":"13","author":"Menon T.","year":"2021","journal-title":"Cureus"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctcp.2020.101214"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S261154"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmedchemlett.7b00138"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2021.1970024"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmedchemlett.0c00009"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2021.116398"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2019.04.002"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15166"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcaa117"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2015.07.065"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.1c00665"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.14.152"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amsu.2021.01.057"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.0c00110"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21866"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol503630z"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-0282-0"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0NJ02656E"},{"key":"e_1_2_8_32_1","unstructured":"World Health Organization.2021 December 15.A report about health. 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