{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:38:19Z","timestamp":1786977499076,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T00:00:00Z","timestamp":1694217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Dengue fever is an acute febrile disease caused by dengue virus (DENV) infection. Over the past 60 years, DENV has spread throughout tropical regions and currently affects more than 50% of the world's population; however, there are as of yet no effective anti\u2010DENV drugs for clinical treatment. A number of research teams have investigated derivatives of glycolipids as possible agents for the inhibition of DENV. Our objective in this research was to study the antiviral effects of trehalose 6\u2010caprate (TMC), trehalose 6\u2010monolaurate (TML), and trehalose 6\u2010monooleate (TMO), based on reports that the corresponding glycosyl, trehalose, reduces the transmission of Zika virus (ZIKV). We also sought to elucidate the molecular mechanisms underlying inhibition using the RNA isolation and reverse transcription\u2010quantitative polymerase chain reaction, western blot analysis, median tissue culture infectious dose (TCID\n                    <jats:sub>50<\/jats:sub>\n                    ) assay, and immunofluorescence assay\u00a0and immunochemistry\u00a0staining, in vitro. This is the first study to demonstrate the TML\u2010induced inhibition of DENV serotype 2 (DENV\u20102) in a dose\u2010dependent manner. The inhibitory effects of TML in the pretreated, cotreated, and full\u2010treated groups were confirmed using time of addition assays. We determined that TML restricted viral binding, entry, replication, and release. We also confirmed the efficacy of TML against three clinical isolates of DENV serotypes 1, 3, and 4 (DENV\u20101, DENV\u20103, and DENV\u20104). The findings obtained in this study identify TML as a promising candidate for the development of drugs to treat DENV infection.\n                  <\/jats:p>","DOI":"10.1002\/ddr.22112","type":"journal-article","created":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T03:34:25Z","timestamp":1694230465000},"page":"1699-1708","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Virucidal activity of trehalose 6\u2010monolaurate against dengue virus in vitro"],"prefix":"10.1002","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2128-2046","authenticated-orcid":false,"given":"Jeng\u2010Wei","family":"Lu","sequence":"first","affiliation":[{"name":"Biotech Research and Innovation Centre, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"},{"name":"The Finsen Laboratory Rigshospitalet\/National University Hospital, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin\u2010Kai","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, National Defense Medical Center Taipei Taiwan, ROC"},{"name":"Department of Pharmacy Tri\u2010Service General Hospital Penghu Branch, National Defense Medical Center Magong City Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen\u2010Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy, National Defense Medical Center Taipei Taiwan, ROC"},{"name":"Institute of Preventive Medicine, National Defense Medical Center New Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guan\u2010Chiun","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Life Science National Taiwan Normal University Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3931-2302","authenticated-orcid":false,"given":"Yi\u2010Jung","family":"Ho","sequence":"additional","affiliation":[{"name":"School of Pharmacy, National Defense Medical Center Taipei Taiwan, ROC"},{"name":"Graduate Institute of Life Sciences, National Defense Medical Center Taipei Taiwan, 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