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MTT assay and the scratch wound healing assay were performed on the osteosarcoma cell line (MG\u201063) to analyze the cytotoxic effect and antimigrative ability of noscapine, respectively. We also observed the antiangiogenic ability of noscapine on zebrafish embryos by analyzing the blood vessels namely the dorsal aorta, and intersegmental vessels\u00a0development at 24, 48, and 72\u2009h postfertilization. Real\u2010time polymerase chain reaction was used to analyze the hypoxia signaling molecules' gene expression in MG\u201063 cells and zebrafish embryos. The findings from the scratch wound healing demonstrated that noscapine stopped MG\u201063 cancer cells from migrating under both hypoxia and normoxia. Blood vessel development and the heart rate in zebrafish embryos were significantly reduced by noscapine under both hypoxia and normoxia which showed the hemodynamics impact of noscapine. Noscapine also downregulated the cobalt chloride (CoCl<jats:sub>2<\/jats:sub>) induced hypoxic signaling molecules'\u00a0gene expression in MG\u201063 cells and zebrafish embryos. Therefore, noscapine may prevent MG\u201063 cancer cells from proliferating and migrating, as well as decrease the formation of new vessels and the production of growth factors linked to angiogenesis in vivo under both normoxic and hypoxic conditions.<\/jats:p>","DOI":"10.1002\/ddr.22195","type":"journal-article","created":{"date-parts":[[2024,5,5]],"date-time":"2024-05-05T12:13:17Z","timestamp":1714911197000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Noscapine modulates hypoxia\u2010induced angiogenesis and hemodynamics: Insights from a zebrafish model investigation"],"prefix":"10.1002","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0855-0088","authenticated-orcid":false,"given":"Jhansi","family":"Nathan","sequence":"first","affiliation":[{"name":"Zebrafish Developmental Biology Laboratory, AUKBC Research Centre for Emerging Technologies Anna University Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabiathul","family":"Shameera","sequence":"additional","affiliation":[{"name":"Zebrafish Developmental Biology Laboratory, AUKBC Research Centre for Emerging Technologies Anna University Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaniha","family":"Sivakumar","sequence":"additional","affiliation":[{"name":"Zebrafish Developmental Biology Laboratory, AUKBC Research Centre for Emerging Technologies Anna University Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soundarya","family":"Rajendran","sequence":"additional","affiliation":[{"name":"Zebrafish Developmental Biology Laboratory, AUKBC Research Centre for Emerging Technologies Anna University Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elumalai","family":"Perumal","sequence":"additional","affiliation":[{"name":"Cancer Genomics Laboratory, Center for Global Health Research, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences Saveetha University Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2013.0901"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2011.11.020"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021076"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24010139"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092280107"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092160207"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920009787522197"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017733"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2403"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12344"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.632662"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9995"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-016-9358-5"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4149\/BLL_2020_007"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2020.104734"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2020.12.007"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.6026\/97320630014236"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2022.173455"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.10.045"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.4529"},{"issue":"5","key":"e_1_2_10_23_1","first-page":"1121","article-title":"Noscapine inhibits hypoxia\u2010mediated HIF\u20101alpha expression andangiogenesis in vitro: A novel function for an old drug","volume":"28","author":"Newcomb E. 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