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In this study, we investigated the anticancer properties of originally synthesized novel indolin\u20102\u2010one derivatives (<jats:italic><jats:bold>6a\u2010d<\/jats:bold><\/jats:italic>) against Malignant Mesothelioma, Breast cancer, and Colon Cancer cells. Our results revealed that all derivatives were effectively delayed cell proliferation by inhibiting the ERK1\/2, AKT, and STAT3 signaling pathways in a concentration\u2010dependent manner. Additionally, these variants induced cell cycle arrest in the S phase, accompanied by elevated levels of p21 and p27 expressions. Derivatives also initiated mitochondrial apoptosis through the upregulation of Bax and downregulation of Bcl\u20102 proteins, leading to the activation of caspase 3 and PARP cleavage in exposed cells. Remarkably, three of the indolin\u20102\u2010one derivatives displayed significant selectivity towards Breast and Colon Cancer cells, with compound <jats:italic><jats:bold>6d<\/jats:bold><\/jats:italic> promising as the most potent and wide spectral one for all cancer cell lines.<\/jats:p>","DOI":"10.1002\/ddr.22229","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T06:14:56Z","timestamp":1719987296000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Preliminary evaluation of antiproliferative and apoptotic activities of novel indolin\u20102\u2010one derivatives"],"prefix":"10.1002","volume":"85","author":[{"given":"Gulseren","family":"Turhal","sequence":"first","affiliation":[{"name":"Department of Molecular Biology &amp; Genetics, Faculty of Science Gebze Technical University Kocaeli 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