{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:20:28Z","timestamp":1777497628626,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T00:00:00Z","timestamp":1724630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nonfunctioning pituitary adenoma (NFPA) is one of the major subtypes of pituitary adenomas (PA) and its primary treatment is surgical resection. However, normal surgery fails to remove lesions completely and there remains in lack of frontline treatment, so the development of new drugs for NFPA is no doubt urgent. Oridonin (ORI) has been reported to have antitumor effects on a variety of tumors, but whether it could exhibit the same effect on NFPA requires to be further investigated. The effects of ORI on pituitary\u2010derived folliculostellate cell line (PDFS) cell viability, colony formation, proliferation ability, migration, and invasion were examined by Cell Counting Kit\u20108, colony formation assay,\u00a05\u2011Ethynyl\u20112'\u2011deoxyuridine proliferation assay, wound\u2010healing assay, and Transwell assay. The differentially expressed genes in the control and ORI\u2010treated groups were screened by transcriptome sequencing analysis and analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment. Cell cycle analysis was performed to detect changes in cell cycle. Annexin V\u2010fluorescein isothiocyanate\/propidium iodide staining was performed to detect apoptosis in ORI\u2010treated cells. Western blot assay was performed to detect Bax, Bcl\u20102, and cleaved Caspase\u20103 protein expression. ORI inhibited PDFS cell viability and significantly suppressed cell proliferation, migration, and invasion. GO and KEGG results showed that ORI was associated with signaling pathways such as cell cycle and apoptosis in PDFS cells. In addition, ORI blocked cells in G2\/M phase and induced apoptosis in PDFS cells. ORI can trigger cell cycle disruption and apoptosis collaboratively in PDFS cells, making it a promising and effective agent for NFPA therapy.<\/jats:p>","DOI":"10.1002\/ddr.22251","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T01:14:27Z","timestamp":1724721267000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Induction of apoptosis by oridonin in nonfunctioning pituitary adenoma cells"],"prefix":"10.1002","volume":"85","author":[{"given":"Hui\u2010Tong","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences Guangdong Pharmaceutical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing\u2010Yi","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences Guangdong Pharmaceutical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong\u2010Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences Guangdong Pharmaceutical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences Guangdong Pharmaceutical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiong\u2010Ming","family":"Luo","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of LifeSciences and Biopharmaceutics Guangdong Pharmaceutical University Guangzhou China"},{"name":"Department of Marine Pharmacy, School of Life Sciences and Biopharmaceutics Guangdong Pharmaceutical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun\u2010Hua","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences Guangdong Pharmaceutical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Xin","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences Guangdong Pharmaceutical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences Guangdong Pharmaceutical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4508-0919","authenticated-orcid":false,"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences Guangdong Pharmaceutical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zong\u2010Ming","family":"Wang","sequence":"additional","affiliation":[{"name":"Pituitary Tumor Center, Department of Neurosurgery, The First Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.85.3.6424"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2018.11.015"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2007.07.001"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.09.040"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.110669"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2015.3186"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/np9008199"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17091395"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2021.109098"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-016-0336-z"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.1031779"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.12140"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.05.006"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2009.06.022"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.19699"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11102-018-0869-3"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29030731"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-018-0030-7"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715762.2012.700515"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-024-11951-1"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6829"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e18046"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28020805"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-023-02702-4"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-06-032250"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.55929"}],"container-title":["Drug Development Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ddr.22251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T08:32:41Z","timestamp":1760344361000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/ddr.22251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,26]]},"references-count":26,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/ddr.22251"],"URL":"https:\/\/doi.org\/10.1002\/ddr.22251","archive":["Portico"],"relation":{},"ISSN":["0272-4391","1098-2299"],"issn-type":[{"value":"0272-4391","type":"print"},{"value":"1098-2299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,26]]},"assertion":[{"value":"2024-05-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22251"}}