{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:31:01Z","timestamp":1760401861020,"version":"build-2065373602"},"reference-count":57,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>\u03b1\u2010Lapachone (aLAP) and \u03b2\u2010lapachone (bLAP) are noteworthy anticancer naphthoquinones. The chemoresistance observed in bladder cancer represents a global health concern, with relation to mutations in the <jats:italic>TP53<\/jats:italic> gene and alterations in the expression of long noncoding RNA (lncRNAs). This study evaluated the effects of aLAP and bLAP on bladder tumor cell lines with different <jats:italic>TP53<\/jats:italic> statuses: RT4 low\u2010grade tumor with wild\u2010type <jats:italic>TP53<\/jats:italic>), T24 and J82 (high\u2010grade tumor with mutation in the <jats:italic>TP53<\/jats:italic> gene). Cytotoxicity was assessed using the MTT reduction method and cell migration by scratch assay, while clonogenic survival and cell cycle were evaluated through cell colony counting and flow cytometry, respectively. The expression of lncRNAs linked to bladder cancer and associated with tumor progression and prognosis (<jats:italic>JHDM1D\u2010AS1<\/jats:italic>, <jats:italic>SBF2\u2010AS1<\/jats:italic>, <jats:italic>CDT\u20102132N18.2<\/jats:italic>, and <jats:italic>RP11\u2010363E7.4<\/jats:italic>) and the <jats:italic>JHDM1D<\/jats:italic> gene was evaluated through RT\u2010qPCR. bLAP demonstrated greater cytotoxicity than aLAP. Its inhibitory effects on clonogenic survival, migration, and the cell cycle were observed in all cell lines and were related to the modulation of lncRNAs expression. A reduction in lncRNA <jats:italic>SBF2\u2010AS1<\/jats:italic> and <jats:italic>JHDM1D<\/jats:italic> gene expression was observed in RT4 cells, accompanied by an increase in lncRNA <jats:italic>RP11\u2010363E7.4<\/jats:italic>. Conversely, in the cells with mutated <jats:italic>TP53<\/jats:italic> (J82), a reduction in <jats:italic>JHDM1D\u2010AS1<\/jats:italic> and <jats:italic>JHDM1D<\/jats:italic> was observed. The downregulation of <jats:italic>JHDM1D\u2010AS1<\/jats:italic> and <jats:italic>SBF2\u2010AS1<\/jats:italic>, along with the upregulation of <jats:italic>RP11\u2010363E7.4<\/jats:italic>, may be associated with the observed inhibition of proliferation and cell migration following bLAP treatment. The antiproliferative effects of bLAP in bladder cancer cells are independent of <jats:italic>TP53<\/jats:italic> statuses, yet occur through a distinct action mechanism, with variations in lncRNAs expression.<\/jats:p>","DOI":"10.1002\/ddr.70128","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T07:16:45Z","timestamp":1752736605000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Beta\u2010Lapachone: Effects on Proliferation, Survival, Migration, Cell Cycle, and lncRNA Modulation in Bladder Cancer Cells With Distinct <i>TP53<\/i> Profiles"],"prefix":"10.1002","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6426-7113","authenticated-orcid":false,"given":"Tatiane Roquete","family":"Amparo","sequence":"first","affiliation":[{"name":"Federal University of Ouro Preto Ouro Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamila de F\u00e1tima da","family":"Anuncia\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Federal University of Ouro Preto Ouro Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamires Cunha","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laboratory of Pain and Signaling, Butantan Institute  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9751-3379","authenticated-orcid":false,"given":"Glenda Nicioli da","family":"Silva","sequence":"additional","affiliation":[{"name":"Federal University of Ouro Preto Ouro Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7330-051X","authenticated-orcid":false,"given":"Geraldo C\u00e9lio","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Federal University of Ouro Preto Ouro Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2022.2151007"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.can-04-0496"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0210635"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.can-07-2964"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12672-024-01173-z"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.12.051"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-4324-2024240053"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e31286"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3514"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22555"},{"issue":"12","key":"e_1_2_10_12_1","first-page":"569","article-title":"p53 Mutations in Bladder Carcinoma Cell Lines","volume":"6","author":"Cooper M. 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