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This study investigates examines the efficacy of curcumin (Cur), a natural bioactive compound, in suppressing inhibiting the proliferation of hepatocellular carcinoma proliferation and reducing lipid accumulation in vitro. HepG2 cells were treated with Cur (1.25\u201310\u2009\u03bcg\/mL) for 24\u201372\u2009h, revealing a dose\u2010 and time\u2010dependent reduction in viability, with an IC50 of 10\u2009\u00b5g\/mL at 72\u2009h. Oil Red O staining demonstrated Cur's lipid\u2010lowering effects, reducing lipid content by 57% at 5\u2009\u00b5g\/mL and 78% at 10\u2009\u00b5g\/mL, suggesting enhanced efficacy at higher concentrations. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT\u2010PCR) analysis revealed that Cur downregulated key lipogenic regulators Peroxisome proliferator\u2010activated receptor gamma (PPAR\u03b3) and CCAAT\/enhancer\u2010binding protein alpha (C\/EBP\u2010\u03b1) by 2.3\u2010 and 1.8\u2010fold, respectively, while modulating 14\u20103\u20103\u03b3\/\u03b2 expression, implicating these pathways in its mechanism. These findings highlight Cur's potential to attenuate hepatic lipid accumulation and cancer cell growth in vitro, warranting further validation in primary hepatocytes and preclinical models to advance its therapeutic prospects for Metabolic Dysfunction\u2010Associated Steatotic Liver Disease (MASLD).<\/jats:p>","DOI":"10.1002\/ddr.70142","type":"journal-article","created":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T10:39:03Z","timestamp":1754649543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Investigating the Effects of Curcumin on Lipid Metabolism and Cell Viability in HepG2 Cells: Potential Therapeutic Implications for Nonalcoholic Fatty Liver Disease"],"prefix":"10.1002","volume":"86","author":[{"given":"Faeze","family":"Qaleban","sequence":"first","affiliation":[{"name":"Islamic Azad University, North Tehran Branch: Hakimiyeh\u2010Tehranpars Tehran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1419-899X","authenticated-orcid":false,"given":"Javad","family":"Mohammadnejad","sequence":"additional","affiliation":[{"name":"Department of Nanobiotechnology and Biomimetic, School of Life Science Engineering College of interdisciplinary Science and Technology University of Tehran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2368-4324","authenticated-orcid":false,"given":"Amin","family":"Daemi","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Cukurova University Adana Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcl\u00fczar","family":"\u00d6zbolat","sequence":"additional","affiliation":[{"name":"Faculty of Health Science Sinop University Sinop Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuf","family":"D\u00f6\u011f\u00fc\u015f","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Cukurova University Adana Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,8]]},"reference":[{"issue":"1","key":"e_1_2_11_2_1","first-page":"1","article-title":"The Ameliorative Effect of Apium graveolens & Curcumin Against Non\u2010Alcoholic Fatty Liver Disease Induced by High Fructose\u2010High Fat Diet in Rats. 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