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In this study, a tetracyclic taxane compound with antitumor activity (taxinine) was extracted and isolated from <jats:italic>Taxus chinensis<\/jats:italic> (<jats:italic>T. chinensis<\/jats:italic>) seeds. It was then formulated into liposomes using lecithin, cholesterol, and D\u2010<jats:italic>\u03b1<\/jats:italic>\u2010tocopheryl polyethylene glycol succinate (TPGS) as excipients to enhance its solubility and antitumor efficacy. The isolation of taxinine was achieved through ultrasound\u2010assisted ethanol extraction, followed by silica gel column chromatography, MTT activity screening, and recrystallization. Afterward, the structure of taxinine was confirmed using nuclear magnetic resonance and mass spectrometry. Taxinine liposomes were prepared via the thin film dispersion method, while the particle size, polydispersity index, zeta potential, and encapsulation efficiency of the nanoliposomes were discovered to be 186.76\u2009\u00b1\u20090.08\u2009nm, 0.226\u2009\u00b1\u20090.012, \u221244.34\u2009\u00b1\u20090.77\u2009mV, and 93.75\u2009\u00b1\u20091.29%, respectively. They also showed good stability with a release rate of 85.77%\u2009\u00b1\u20092.43% in phosphate\u2010buffered solution (PBS, pH 7.4). Toxicity tests conducted on zebrafish larvae indicated that taxinine liposomes were safe in vivo. Tissue distribution study showed that the concentration of taxinine liposomes increased to varying degrees in tissues (especially liver). In vitro experiments demonstrated that taxinine liposomes significantly enhanced the inhibitory effect of taxinine on HepG2 cell growth. Overall, the nanoliposomal formulation improved the anti\u2010liver cancer activity of taxinine, thus suggesting its potential as a therapeutic agent.<\/jats:p>","DOI":"10.1002\/ddr.70143","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T10:59:57Z","timestamp":1757329197000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Isolation, Purification, and Preparation of Taxinine\u2010Loaded Liposomes for Improved Anti\u2010Hepatocarcinogenic Activity"],"prefix":"10.1002","volume":"86","author":[{"given":"Qilong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug\/Gene Delivery and Tissue Engineering, Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug\/Gene Delivery and Tissue Engineering, Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7740-1899","authenticated-orcid":false,"given":"Qinyang","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug\/Gene Delivery and Tissue Engineering, Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug\/Gene Delivery and Tissue Engineering, Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug\/Gene Delivery and Tissue Engineering, Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjie","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug\/Gene Delivery and Tissue Engineering, Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingyuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug\/Gene Delivery and Tissue Engineering, Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug\/Gene Delivery and Tissue Engineering, Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elmurat","family":"Toreniyazov","sequence":"additional","affiliation":[{"name":"Tashkent State Agricultural University (Nukus Branch) Nukus Uzbekistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangnan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug\/Gene Delivery and Tissue Engineering, Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Adu\u2010Frimpong","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Forensic Sciences, School Chemical and Biochemical Sciences C. 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