{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T07:16:42Z","timestamp":1779175002738,"version":"3.51.4"},"reference-count":69,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T00:00:00Z","timestamp":1758412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T00:00:00Z","timestamp":1758412800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The chlorophyll\u2010derived diterpenoid phytol (PHY) is evident for its diverse biological effects, including neuroprotective activities in experimental animals. This study aims at the evaluation of anticonvulsant effects along with the possible mechanism of action of PHY through animal and\n                    <jats:italic>in silico<\/jats:italic>\n                    studies. For this, we performed pentylenetetrazole (PTZ)\u2010induced convulsion tests in chicks and\n                    <jats:italic>in silico<\/jats:italic>\n                    studies against GABA\n                    <jats:sub>A<\/jats:sub>\n                    receptor subunits and voltage\u2010gated sodium channel (VGSC) receptors. PHY was tested at 25, 50, and 75\u2009mg\/kg with or without the standard drugs diazepam (DZP: 5\u2009mg\/kg) and carbamazepine (CAR: 80\u2009mg\/kg) using a vehicle as a control. Acute oral toxicity was evaluated in chicks per OECD guidelines by administering PHY (500\u20132000\u2009mg\/kg, p.o.) and monitoring for 48\u2009h for mortality, toxicological signs, and behavioral changes. PHY exhibited a dose\u2010dependent anticonvulsant effect, significantly increasing latency and reducing convulsion frequency and duration at 75\u2009mg\/kg. PHY (75\u2009mg\/kg) combined with CAR and DZP showed the most potent reduction in convulsion frequency and duration, indicating a synergistic effect. Acute toxicity tests in chicks confirmed safety up to 2000 mg\/kg.\n                    <jats:italic>In silico<\/jats:italic>\n                    studies demonstrated that PHY had good binding affinity with both the GABA\n                    <jats:sub>A<\/jats:sub>\n                    receptor (\u20136.5\u2009kcal\/mol) and VGSC (\u20137.0\u2009kcal\/mol), potentially contributing to its anticonvulsant action through GABAergic and sodium channel modulation. PHY showed significant anticonvulsant activity, likely via GABA\n                    <jats:sub>A<\/jats:sub>\n                    and VGSC modulation, warranting further studies to clarify its mechanisms and assess its adjunct potential in drug\u2010resistant convulsion management.\n                  <\/jats:p>","DOI":"10.1002\/ddr.70162","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T02:54:32Z","timestamp":1758509672000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Phytol Enhances Anticonvulsant Effect of Carbamazepine and Diazepam Against PTZ\u2010Induced Convulsion in Chicks, Possibly Through Voltage\u2010Gated Sodium Channel and GABA\n                    <sub>A<\/sub>\n                    Interaction Pathways"],"prefix":"10.1002","volume":"86","author":[{"given":"Md. Torequl","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd. Gopalganj Bangladesh"},{"name":"Pharmacy Discipline Khulna University Khulna Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoyaeb","family":"Ahammed","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd. Gopalganj Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7560-4041","authenticated-orcid":false,"given":"Md. Sakib","family":"Al Hasan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7096-0221","authenticated-orcid":false,"given":"Mohammad Y.","family":"Alshahrani","sequence":"additional","affiliation":[{"name":"Central labs King Khalid University Abha Saudi Arabia"},{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences King Khalid University Abha Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0672-6581","authenticated-orcid":false,"given":"Raihan","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd. Gopalganj Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3705-6967","authenticated-orcid":false,"given":"Salehin","family":"Sheikh","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd. Gopalganj Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7390-056X","authenticated-orcid":false,"given":"Emon","family":"Mia","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fazley","family":"Rohan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imam Hossen","family":"Rakib","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. 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