{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:01:56Z","timestamp":1792202516619,"version":"4.4.0"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon, characterized by persistent mucosal inflammation and epithelial barrier disruption. This study investigated the therapeutic efficacy of Scopoletin, a natural coumarin derivative with established anti\u2010inflammatory and antioxidant properties, in a DSS\u2010induced colitis model in Balb\/c mice. A total of five experimental groups were established: a normal control, a DSS+ vehicle group, two Scopoletin\u2010treated groups (10 and 30\u2009mg\/kg), and a reference group treated with Sulfasalazine (200\u2009mg\/kg). Network pharmacology analyses identified key inflammatory and immune\u2010regulatory pathways potentially modulated by Scopoletin.\n                    <jats:italic>In vivo<\/jats:italic>\n                    assessments encompassed body weight monitoring, DAI scoring, colon length measurement, and histopathological evaluation using H&amp;E, PAS, and Alcian blue staining. Scopoletin (30\u2009mg\/kg) treatment significantly ameliorated clinical and histological manifestations of colitis, including body weight loss and colonic shortening. Mechanistically, Scopoletin (30\u2009mg\/kg) attenuated the expression of pro\u2010inflammatory cytokines such as TNF\u2010\u03b1 and IL\u20101\u03b2, suppressed NF\u2010\u03baB activation, MMP\u20109, COX\u20102 and enhanced the Nrf2 expression, leading to upregulation of antioxidant enzymes HO\u20101 and NQO1. Notably, Scopoletin (30\u2009mg\/kg) restored the expression of tight junction proteins such as Occludin and ZO\u20101, indicating reinforcement of epithelial barrier integrity. These findings demonstrated that Scopoletin protects against UC by suppressing inflammation, enhancing antioxidant defenses, and preserving mucosal barrier integrity, highlighting its potential as a therapeutic candidate for UC.\n                  <\/jats:p>","DOI":"10.1002\/ddr.70165","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T08:55:38Z","timestamp":1758790538000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Scopoletin Mitigates DSS\u2010Induced Ulcerative Colitis by Attenuating NF\u2010\u03baB\/MMP\u20109 Mediated Inflammation and\u00a0Activating the Nrf2 Pathway to Preserve Colonic Barrier Integrity"],"prefix":"10.1002","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2578-9741","authenticated-orcid":false,"given":"A","family":"Gowtham","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Raebareli (NIPER\u2010R), Transit Campus, Bijnor\u2010Sisendi Road, Sarojini Nagar, Near CRPF Base Camp Lucknow (UP)\u2010 226002"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tushar","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Raebareli (NIPER\u2010R), Transit Campus, Bijnor\u2010Sisendi Road, Sarojini Nagar, Near CRPF Base Camp Lucknow (UP)\u2010 226002"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarh","family":"Gungha","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Raebareli (NIPER\u2010R), Transit Campus, Bijnor\u2010Sisendi Road, Sarojini Nagar, Near CRPF Base Camp Lucknow (UP)\u2010 226002"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8519-274X","authenticated-orcid":false,"given":"Ravinder K.","family":"Kaundal","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Raebareli (NIPER\u2010R), Transit Campus, Bijnor\u2010Sisendi Road, Sarojini Nagar, Near CRPF Base Camp Lucknow (UP)\u2010 226002"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0249544"},{"issue":"6","key":"e_1_2_10_3_1","first-page":"778","article-title":"Activation of Nuclear Factor\u2010Kappa B and Expression of Cytokine in Intestinal Epithelia Stimulated by TNF\u2010alpha","volume":"35","author":"Bai A. 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