{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T16:06:10Z","timestamp":1784995570446,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Bladder and ovarian cancers impose a significant burden on healthcare systems due to their high incidence, mortality rates, and the challenges associated with early diagnosis. Current chemotherapy regimens, which typically involve combinations of drugs, often cause severe side effects that negatively impact patient adherence and treatment efficacy. Recently, studies have explored the use of herbal medicines to mitigate the adverse effects of chemotherapy. One such herbal compound is ursolic acid (UA), a triterpene known for its anti\u2010inflammatory, antioxidant, and antitumor properties. This study aimed to evaluate the effects of UA on bladder and ovarian cancer cells harboring\n                    <jats:italic>TP53<\/jats:italic>\n                    mutations through various assays, including cytotoxicity, clonogenic survival, cell migration, morphological changes, apoptosis, cell cycle analysis,\n                    <jats:italic>JHDM1D<\/jats:italic>\n                    expression and selectivity using MRC\u20105 cells, along with in silico evaluation. The treatment demonstrated selectivity for tumoral cells and significant antiproliferative effects in both cell types, leading to decreased cell viability, reduced colony\u2010forming ability, inhibited cell migration, morphological changes characteristic of cell death, and increased expression of\n                    <jats:italic>JHDM1D<\/jats:italic>\n                    . In conclusion, UA exhibited antiproliferative activity against bladder and ovarian cancer cell lines with different\n                    <jats:italic>TP53<\/jats:italic>\n                    mutation sites, suggesting its potential as a promising therapeutic alternative. Moreover, our study demonstrated for the first time the presence of UA in the species\n                    <jats:italic>F. formosa<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/ddr.70172","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T09:17:03Z","timestamp":1758878223000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antiproliferative Effects of the Triterpene Ursolic Acid Natural Product in Bladder and Ovarian Tumor Cell Lines"],"prefix":"10.1002","volume":"86","author":[{"given":"L\u00eddia Walter de Paula e","family":"Silva","sequence":"first","affiliation":[{"name":"School of Pharmacy Federal University of Ouro Preto Ouro Preto MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5584-3609","authenticated-orcid":false,"given":"Tamires Cunha","family":"Almeida","sequence":"additional","affiliation":[{"name":"Pain and Signaling Laboratory, Butantan Institute S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariane Ster da Silva","family":"Teixeira","sequence":"additional","affiliation":[{"name":"School of Pharmacy Federal University of Ouro Preto Ouro Preto MG Brazil"},{"name":"Postgraduate Program in Pharmaceutical Siences Federal University of Ouro Preto  MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara Maria Villela","family":"Cerrutti","sequence":"additional","affiliation":[{"name":"School of Pharmacy Federal University of Ouro Preto Ouro Preto MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6524-9200","authenticated-orcid":false,"given":"L\u00edvia da Cunha","family":"Agostini","sequence":"additional","affiliation":[{"name":"School of Pharmacy Federal University of Ouro Preto Ouro Preto MG Brazil"},{"name":"Postgraduate Program in Pharmaceutical Siences Federal University of Ouro Preto  MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7330-051X","authenticated-orcid":false,"given":"Geraldo Celio","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"School of Pharmacy Federal University of Ouro Preto Ouro Preto MG Brazil"},{"name":"Postgraduate Program in Pharmaceutical Siences Federal University of Ouro Preto  MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9751-3379","authenticated-orcid":false,"given":"Glenda Nicioli","family":"da Silva","sequence":"additional","affiliation":[{"name":"School of Pharmacy Federal University of Ouro Preto Ouro Preto MG Brazil"},{"name":"Postgraduate Program in Pharmaceutical Siences Federal University of Ouro Preto  MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10654-016-0138-6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urolonc.2018.02.017"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.22363"},{"issue":"8","key":"e_1_2_8_5_1","first-page":"418","article-title":"Carboplatin\/Paclitaxel Induction in Ovarian Cancer: The Finer Points","volume":"32","author":"Boyd L. 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