{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:26:53Z","timestamp":1788964013824,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to investigate the protective effect of curcumin against sepsis\u2010associated acute kidney injury (SA\u2010AKI) in vitro and in vivo, and to clarify the role of ferroptosis in this protection. Human renal tubular epithelial cell (RTEC) line HK\u20102 was stimulated with lipopolysaccharide (LPS). Ferroptosis was further induced with erastin. In vivo, a rat SA\u2010AKI model was produced by cecal ligation and puncture (CLP) and allocated to the Sham, sepsis, and curcumin (Cur)\u2009+\u2009sepsis (Sep) groups. Glutathione (GSH), malondialdehyde (MDA), and pro\u2010inflammatory cytokines were quantified by ELISA. Western blot analysis was used to evaluate the ferroptosis\u2010related proteins, including long\u2010chain acyl\u2010coenzyme synthetases 4 (ACSL4) and glutathione peroxidase 4 (GPX4). Apoptosis of RTECs was assessed with TUNEL staining, and ultrastructural changes were examined by transmission electron microscopy (TEM). Compared with the Sepsis group, the Cur\u2009+\u2009Sep group showed significantly lower Paller damage scores, reduced Scr, BUN, TNF\u2010\u03b1, IL\u20101\u03b2, IL\u20106, MDA, Fe\n                    <jats:sup>2+<\/jats:sup>\n                    levels, and ACSL4 protein expression (all\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Conversely, GSH and GPX4 levels were significantly elevated in the Cur + Sep group (both\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). TUNEL staining revealed fewer apoptotic RTECs in the Cur\u2009+\u2009Sep group compared with the Sepsis group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). TEM demonstrated swollen mitochondria with condensed membranes and vanished cristae in the sepsis group, changes that were markedly alleviated by curcumin. In HK\u20102 cells, erastin abolished curcumin's protective effect against LPS\u2010induced injury. Curcumin attenuates SA\u2010AKI, likely by suppressing inflammation and ferroptosis via the ACSL4\/GPX4 signaling pathway.\n                  <\/jats:p>","DOI":"10.1002\/ddr.70181","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T07:25:29Z","timestamp":1761549929000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Curcumin Alleviates Sepsis\u2010Associated Acute Kidney Injury Potentially by Inhibiting Ferroptosis Through the ACSL4\/GPX4 Signaling Pathway"],"prefix":"10.1002","volume":"86","author":[{"given":"Lifeng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine The First Affiliated Hospital of Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nannan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine The First Affiliated Hospital of Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine The First Affiliated Hospital of Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Die","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine The First Affiliated Hospital of Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongning","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine The First Affiliated Hospital of Dalian Medical University Dalian Liaoning 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