{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T02:48:57Z","timestamp":1788922137420,"version":"build-2803163510"},"reference-count":63,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Angelicin (AGN), an angular furocoumarin, exhibits notable anti\u2010inflammatory and biological activities. However, its potential for managing pain and diarrhea, and the underlying mechanisms, remain poorly explored. This study aimed to investigate the analgesic and antidiarrheal properties of AGN and elucidate its possible mechanisms of action through in vivo and\n                    <jats:italic>in silico<\/jats:italic>\n                    approaches. Mice received AGN (2.5, 5, and 10\u2009mg\/kg, i.p), DFS (25\u2009mg\/kg, p.o), LOP (3\u2009mg\/kg p.o), and BSS (10\u2009mg\/kg, p.o), after 30\u2009min of administration, for an analgesic test; 0.7% acetic acid at 10\u2009mL\/kg (i.p) induced writhing, observed latency, and number of writhing. Antidiarrheal activity was assessed by using castor oil (0.5\u2009mL, p.o) induced diarrhea in mice, observing latency and number of diarrhea secretions over 3\u2009h. Additionally, Molecular docking studies were performed to analyze AGN's interactions with cyclooxygenase (COX\u20101\/2) enzymes and the \u00b5\u2010opioid receptor (MOR). Drug\u2010likeness and toxicity profiles were predicted using SwissADME and ProTox\u2010III. AGN demonstrated significant, dose\u2010dependent analgesic and antidiarrheal effects. Its combination with standard drugs (DFS, LOP, BSS) showed enhanced efficacy. Molecular docking revealed strong binding affinities of AGN for COX\u20101 (\u22127.4\u2009kcal\/mol), COX\u20102 (\u22128.0\u2009kcal\/mol), and MOR (\u22127.1\u2009kcal\/mol). Pharmacokinetic predictions indicated good drug\u2010likeness, and toxicity profiling suggested a favorable safety margin. These results strongly suggest that AGN is a promising dual\u2010action therapeutic candidate for pain and diarrhea, likely mediated through COX inhibition and MOR interaction. Further studies are warranted to validate these mechanisms and develop optimized AGN\u2010based formulations for clinical translation.\n                  <\/jats:p>","DOI":"10.1002\/ddr.70201","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T10:56:57Z","timestamp":1764154617000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploring the Analgesic and Antidiarrheal Properties of Angelicin Through Cyclooxygenase Inhibition and \u039c\u2010Opioid Receptor Interaction: In Vivo and In Silico Studies"],"prefix":"10.1002","volume":"86","author":[{"given":"Sourav","family":"Bagchi","sequence":"first","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipu","family":"Bishwas","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Pabna University of Science and Technology Pabna Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1801-1928","authenticated-orcid":false,"given":"Mst Muslima","family":"Khatun","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Dhaka Bangladesh"},{"name":"Phytochemistry and Biodiversity Research Laboratory BioLuster Research Center Ltd. Gopalganj Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Most. Israt Jahan","family":"Oni","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Dhaka Bangladesh"},{"name":"Phytochemistry and Biodiversity Research Laboratory BioLuster Research Center Ltd. Gopalganj Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6179-9720","authenticated-orcid":false,"given":"Md. Shimul","family":"Bhuia","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University Gopalganj Dhaka Bangladesh"},{"name":"Phytochemistry and Biodiversity Research Laboratory BioLuster Research Center Ltd. 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