{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T04:05:31Z","timestamp":1783569931283,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Glioblastoma is the most common and aggressive malignant brain tumor, characterized by poor response to current therapies and inevitable recurrence. Novel therapeutic strategies are urgently needed.\n                    <jats:italic>Lansbermin\u2010I<\/jats:italic>\n                    , a disintegrin isolated from the venom of\n                    <jats:italic>Porthidium lansbergii lansbergii<\/jats:italic>\n                    , was purified, sequenced, structurally modeled, and evaluated for its antitumor potential against the human Glioblastoma cell line T98G. Cytotoxicity assays revealed a dose\u2010dependent effect, with\n                    <jats:italic>Lansbermin\u2010I<\/jats:italic>\n                    inducing up to 38.1% cell death at 100\u2009\u03bcg\/mL, surpassing the activity of temozolomide (34.9%) while exhibiting lower toxicity on non\u2010tumorigenic human astrocytes (28% vs. 37%).\n                    <jats:italic>Lansbermin\u2010I<\/jats:italic>\n                    also inhibited T98G adhesion to fibronectin by nearly 80% (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001), suggesting interference with integrin\u2010mediated interactions. Flow cytometry demonstrated a significant increase in apoptotic cells (60% vs. 36% in control) and cell cycle arrest at the G1 phase. In silico docking studies supported a strong interaction of\n                    <jats:italic>Lansbermin\u2010I<\/jats:italic>\n                    with integrin \u03b1v\u03b23, comparable to that of fibronectin, reinforcing its potential mechanism of action through the disruption of adhesion and survival signaling. Collectively, these findings highlight\n                    <jats:italic>Lansbermin\u2010I<\/jats:italic>\n                    as a promising selective prototype for Glioblastoma therapy. Further studies are warranted to elucidate its molecular targets and evaluate its efficacy in preclinical models.\n                  <\/jats:p>","DOI":"10.1002\/ddr.70218","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T11:49:07Z","timestamp":1766749747000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Lansbermin\u2010I<\/i>\n                    : A Snake Venom Disintegrin With Selective Cytotoxicity and Anti\u2010Adhesive Effects Against Glioblastoma Cells"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4293-4659","authenticated-orcid":false,"given":"Javier","family":"Orozco\u2010Mera","sequence":"first","affiliation":[{"name":"Grupo de Nutrici\u00f3n, Facultad de Salud Universidad del Valle Cali Valle de Cauca Colombia"},{"name":"Department of Neurosurgery Cl\u00ednica Imbanaco Cali Valle del Cauca 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