{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:12:39Z","timestamp":1786119159101,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study aimed to explore the therapeutic effect and underlying mechanism of \u03b17 nicotinic acetylcholine receptor (\u03b17nAChR) mediated by adeno\u2010associated virus serotype 9 (AAV9) on dextran sulfate sodium (DSS)\u2010induced ulcerative colitis (UC) in mice. Male C57BL\/6 mice were randomly divided into the blank control group, model group, AAV\u2010\u03b17nAChR low\/medium\/high\u2010dose groups, and AAV\u2010GFP group. A chronic UC model was established, with interventions administered via intraperitoneal injection. Meanwhile, an acute UC model was set up, together with control groups treated with the \u03b17nAChR antagonist methyllycaconitine (MLA) and 5\u2010aminosalicylic acid (5\u2010ASA). A series of indicators were detected, including body weight, Disease Activity Index (DAI), colonic pathological changes, inflammatory factors, pathway\u2010related proteins, and T\u2010regulatory (T\u2010reg)\/T helper 17 (Th\u201017) cell balance. Results demonstrated that AAV9\u2010\u03b17nAChR ameliorated UC symptoms in mice in a dose\u2010dependent manner: it relieved body weight loss and hematochezia, restored colon length and spleen weight, and alleviated colonic mucosal damage. Furthermore, it activated the cholinergic anti\u2010inflammatory pathway (CAP), inhibited the NF\u2010\u03baB\/NLRP3 inflammatory axis, repaired the intestinal barrier (by upregulating ZO\u20101 and occludin), and restored the T\u2010reg\/Th\u201017 immune balance. The therapeutic efficacy of the high\u2010dose AAV9\u2010\u03b17nAChR group was superior to that of the 5\u2010ASA group, while MLA could suppress its therapeutic effect. This study preliminarily clarified the multi\u2010target mechanism of AAV9\u2010\u03b17nAChR in treating UC, providing an experimental foundation for the clinical gene therapy of UC.<\/jats:p>","DOI":"10.1002\/ddr.70240","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T20:52:59Z","timestamp":1770238379000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["AAV9\u2010Delivered \u03b17nAChR Ameliorates DSS\u2010Induced Murine Ulcerative Colitis by Activating CAP Pathway, Inhibiting NF\u2010\u03baB\/NLRP3 Axis and Restoring Intestinal Barrier and T\u2010reg\/Th\u201017 Homeostasis"],"prefix":"10.1002","volume":"87","author":[{"given":"Rui","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Engineering Guangzhou College of Technology and Business Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qincheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Baichuan Animal Hospital Foshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Bi","sequence":"additional","affiliation":[{"name":"School of Engineering Guangzhou College of Technology and Business Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/RPS.RPS_46_24"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.4c00784"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v31.i7.96459"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ibd\/izaf006"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2302982"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ueg2.12714"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26073210"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCHEARTFAILURE.112.971325"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.117337"},{"issue":"2","key":"e_1_2_12_11_1","first-page":"368","article-title":"Regulation of Mild Moxibustion on Non\u2010Neuronal Cholinergic System in Ulcerative Colitis Rats","volume":"45","author":"Guangbin P.","year":"2025","journal-title":"Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.2478\/jvetres-2024-0066"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43556-024-00207-w"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2024.1490300"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2024.230"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2407766"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/dom.16060"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2025.1523773"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2024.102521"},{"issue":"1","key":"e_1_2_12_20_1","article-title":"AAV\u2010Mediated Delivery of Plakophilin\u20102a Arrests Progression of Arrhythmogenic Right Ventricular Cardiomyopathy in Murine Hearts: Preclinical Evidence Supporting Gene Therapy in Humans","volume":"17","author":"Opbergen C. 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