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A suitable anti\u2010leishmanial medication that is acceptable in terms of price and safety is currently being researched. In light of this, the current study examined the effectiveness of imidazoquinoline based selective Toll\u2010like receptor (TLR)\u20107 agonists as anti\u2010leishmanial agents. A library of TLR7 agonists was evaluated for anti\u2010leishmanial efficacy against experimental visceral leishmaniasis (VL). Among all the agonists, 2\u2010butyl\u20101\u2010(3,4\u2010dimethoxybenzyl)\u20101\n                    <jats:italic>H<\/jats:italic>\n                    \u2010imidazo[4,5\u2010\n                    <jats:italic>c<\/jats:italic>\n                    ]quinolin\u20104\u2010amine (\n                    <jats:bold>7<\/jats:bold>\n                    ) and its niosomal formulation\n                    <jats:bold>18<\/jats:bold>\n                    exhibited better activity than Miltefosine. These were then further studied against\n                    <jats:italic>Leishmania donovani<\/jats:italic>\n                    for their ability to arrest the parasites at sub G\n                    <jats:sub>0<\/jats:sub>\n                    \/G\n                    <jats:sub>1<\/jats:sub>\n                    phase of cell cycle. The selected TLR7 agonists depicted their ability to arrest higher percentage of parasites in comparison to the standard antileishmanial drugs. Similar observations were seen in case of reactive oxygen species (ROS) generation where parasites treated with the lead TLR7 agonist produced higher ROS than the untreated parasites. These results suggest that TLR7 agonist\n                    <jats:bold>7<\/jats:bold>\n                    and its niosomal formulation\n                    <jats:bold>18<\/jats:bold>\n                    could be the promising hits for development of a novel drug treating VL. However additional in\u2010vivo studies could aid in their future advancement.\n                  <\/jats:p>","DOI":"10.1002\/ddr.70250","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T09:07:14Z","timestamp":1771232834000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Novel Imidazoquinoline Based TLR7 Agonist Induces Antileishmanial Efficacy\u2010An In Vitro Study"],"prefix":"10.1002","volume":"87","author":[{"given":"Shivani","family":"Thakur","sequence":"first","affiliation":[{"name":"Parasitology Laboratory, Department of Zoology Panjab University Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kushvinder","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Centre of Advanced Studies in Chemistry Panjab University Chandigarh 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