{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T15:53:21Z","timestamp":1793116401181,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T00:00:00Z","timestamp":1774742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T00:00:00Z","timestamp":1774742400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A new series of aliphatic\u2010substituted benzimidazole derivatives was synthesized and structurally characterized to evaluate their potential anticancer activity. Among the synthesized compounds, compound 4 exhibited the most potent cytotoxic effects against MCF\u20107 and MDA\u2010MB\u2010231 breast cancer cell lines, with IC\u2085\u2080 values comparable to those of cisplatin, while displaying lower toxicity toward normal breast epithelial cells (MCF\u201010A). Flow cytometric analysis revealed that treatment with compound 4 resulted in significant accumulation of cells in the S phase, indicating inhibition of DNA synthesis and replication. Furthermore, Annexin V\/PI double\u2010staining analysis demonstrated a marked increase in both early and late apoptotic cell populations, confirming the activation of apoptotic pathways. Molecular docking studies supported these experimental findings by revealing strong interactions of compound 4 with key regulatory proteins involved in apoptosis and cell cycle progression, including Bcl\u20102, Bcl\u2010xL, CDK2, and Cyclin E. The compound exhibited the highest binding affinity toward CDK2 (\u2013164.055\u2009kcal\/mol), forming hydrogen bonds with critical residues (LEU134, ASP145, GLN131, and LYS33) within the ATP\u2010binding pocket, suggesting potential inhibition of kinase activity. Interactions with Bcl\u20102 and Bcl\u2010xL occurred within the BH3\u2010binding grooves, which may impair their anti\u2010apoptotic functions and promote mitochondrial\u2010mediated apoptosis. Collectively, the in vitro and in silico results indicate that this newly synthesized benzimidazole derivative exerts its anticancer effects through a dual mechanism involving cell cycle arrest and apoptosis induction. The selective cytotoxicity and multitarget interaction profile of compound 4 highlight its potential as a promising lead compound for the development of novel therapeutic agents against breast cancer.<\/jats:p>","DOI":"10.1002\/ddr.70267","type":"journal-article","created":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T16:46:13Z","timestamp":1774802773000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Synthesis, Characterization, and Biological Evaluation of Aliphatic\u2010Substituted Benzimidazole Derivatives: Induction of Apoptosis, Cell Cycle Arrest, and Molecular Docking in Breast Cancer Cells"],"prefix":"10.1002","volume":"87","author":[{"given":"Murat","family":"Keser","sequence":"first","affiliation":[{"name":"Department of Medical Oncology Medicana International \u0130zmir Hospital \u0130zmir 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