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This study investigates the repurposing of palbociclib (PB) and disulfiram (DS), alongside sodium selenite (SS), as potential therapeutic strategies. Using cell lines, we assessed antiproliferative effects via Sulforhodamine B, colony formation, and wound healing assays. Mechanisms of action were explored through protein expression of PARP\u20101 (apoptosis) and LC3\u03b2 (autophagy), qPCR for stem cell markers, and ROS quantification. Finally, antitumor and anti\u2010angiogenic efficacy was validated using the in ovo chicken chorioallantoic membrane (CAM) assay. Results demonstrated that all three compounds inhibited proliferation, migration, and spheroid growth while inducing apoptosis and autophagy. Notably, SS and PB elevated ROS levels, triggering parthanatos\u2010mediated cell death via AIF nuclear translocation. SS also exhibited significant anti\u2010angiogenic activity. Xenograft CAM models confirmed the in vivo efficacy of SS, PB, and DS against RD and MG63 cells. These findings suggest that SS, PB, and DS are promising candidates for pediatric sarcoma treatment, particularly as maintenance therapies to prevent relapse following conventional radical treatment.<\/jats:p>","DOI":"10.1002\/ddr.70304","type":"journal-article","created":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T21:00:57Z","timestamp":1777842057000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antitumor Activity of Sodium Selenite, Palbociclib, and Disulfiram Against Osteosarcoma and Rhabdomyosarcoma Cell Lines"],"prefix":"10.1002","volume":"87","author":[{"given":"Mar\u00eda \u00c1ngeles","family":"Chico","sequence":"first","affiliation":[{"name":"Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM) Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada, ibs.GRANADA University of Granada Granada Spain"},{"name":"Department of Anatomy and Embryology, Faculty of Medicine University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Doello","sequence":"additional","affiliation":[{"name":"Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM) Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada, ibs.GRANADA University of Granada Granada Spain"},{"name":"Department of Anatomy and Embryology, Faculty of Medicine University of Granada Granada Spain"},{"name":"Medical Oncology Service, Virgen de las Nieves Hospital Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8409-5235","authenticated-orcid":false,"given":"Raul","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM) Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada, ibs.GRANADA University of Granada Granada Spain"},{"name":"Department of Anatomy and Embryology, Faculty of Medicine University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM) Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada, ibs.GRANADA University of Granada Granada Spain"},{"name":"Department of Anatomy and Embryology, Faculty of Medicine University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3990-806X","authenticated-orcid":false,"given":"Consolaci\u00f3n","family":"Melguizo","sequence":"additional","affiliation":[{"name":"Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM) Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada, ibs.GRANADA University of Granada Granada Spain"},{"name":"Department of Anatomy and Embryology, Faculty of Medicine University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Mesas","sequence":"additional","affiliation":[{"name":"Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM) Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada, ibs.GRANADA University of Granada Granada Spain"},{"name":"Department of Anatomy and Embryology, Faculty of Medicine University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4303-7746","authenticated-orcid":false,"given":"Jose","family":"Prados","sequence":"additional","affiliation":[{"name":"Institute of Biopathology and Regenerative Medicine (IBIMER), Center of Biomedical Research (CIBM) Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada, ibs.GRANADA University of Granada Granada Spain"},{"name":"Department of Anatomy and Embryology, Faculty of Medicine University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2019.09.012"},{"issue":"5","key":"e_1_2_11_3_1","article-title":"Serum Osteopontin as a Potential Marker for Metastasis and Prognosis in Primary Osteogenic Sarcoma: A Systematic Review","volume":"16","author":"Agrawal A. 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