{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:06:17Z","timestamp":1789027577939,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Among adolescents worldwide, osteosarcoma (OS) is one of the most frequently occurring cancers. DSCAM\u2010AS1, a recognized lncRNA, has been noted for its abnormal expression in the development of certain cancers, but its role in OS is still unclear. Quantification of DSCAM\u2010AS1, miR\u2010211\u20105p, and PDCD6 expression was carried out via qRT\u2010PCR or western blotting in human osteosarcoma cell lines (HOS, MG63, U2OS, SaOS\u20102) and a normal osteoblastic cell line (hFOB 1.19) using quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) for RNA detection and western blotting for protein detection, respectively. Cellular proliferation, apoptosis, migration, and invasion in OS cell models were comprehensively evaluated through a combination of experimental approaches, including EdU incorporation assay, CFSE labeling coupled with flow cytometric analysis, as well as transwell invasion and migration assays. To shed light on the molecular mechanisms of interactions among pertinent RNA molecules, researchers conducted luciferase reporter assays, RNA pull\u2010down experiments, and RIP assays. Expression of DSCAM\u2010AS1, miR\u2010211\u20105p, and PDCD6 was analyzed based on the GEO database, and their correlations were evaluated. The levels of DSCAM\u2010AS1and PDCD6 were predominantly overexpressed, while miR\u2010211\u20105p was apparently lowly expressed in OS cells. Functional loss\u2010of\u2010function experiments demonstrated that silencing (knockdown) of DSCAM\u2010AS1 expression substantially diminished OS cell proliferation, migration, and invasion, while simultaneously promoting cellular apoptosis in vitro. Mechanistically. DSCAM\u2010AS1 acted as an upstream factor for miR\u2010211\u20105p and could increase the expression of miR\u2010211\u20105p\u2010targeted PDCD6. DSCAM\u2010AS1 facilitated the advancement of OS by increasing PDCD6 levels through miR\u2010211\u20105p sponging. This observation could shed light on a novel therapeutic target option for OS.<\/jats:p>","DOI":"10.1002\/ddr.70360","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:40:03Z","timestamp":1786977603000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DSCAM\u2010AS1 Facilitates Osteosarcoma Progression via Regulating miR\u2010211\u20105p\/PDCD6 Axis"],"prefix":"10.1002","volume":"87","author":[{"given":"Qi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunzheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longyan","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8841-3603","authenticated-orcid":false,"given":"Qiuliang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.07.031"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2019.1646562"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-018-0674-0"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.09.028"},{"issue":"1","key":"e_1_2_10_6_1","doi-asserted-by":"crossref","DOI":"10.1177\/2058738418820743","article-title":"LncRNA SNHG3 Promotes Cell Growth by Sponging miR\u2010196a\u20105p and Indicates the Poor Survival in Osteosarcoma","volume":"33","author":"Chen J.","year":"2019","journal-title":"International Journal of Immunopathology and Pharmacology"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2020.08.004"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-018-9763-8"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-022-02736-2"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncrna.2018.10.001"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.758513"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.ijmr_1588_22"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(18)30821-0"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1539"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3233\/BD-180351"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc18109"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.04.203"},{"key":"e_1_2_10_18_1","first-page":"1087","article-title":"Long Noncoding RNA DSCAM\u2010AS1 Functions as an Oncogene in Non\u2010Small Cell Lung Cancer by Targeting BCL11A","volume":"23","author":"Liao J.","year":"2019","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-014-2081-z"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.03.154"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01496-3"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-025-01179-x"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12791"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-020-01342-4"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-018-0654-4"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-10-5203-3_7"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S205544"},{"key":"e_1_2_10_28_1","first-page":"83","article-title":"MicroRNAs: Processing, Maturation, Target Recognition and Regulatory Functions","volume":"3","author":"Shukla G. 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