{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:06:16Z","timestamp":1789027576254,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601273"],"award-info":[{"award-number":["81601273"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diabetes mellitus (DM) related erectile dysfunction (ED) is a common complication in males. We aim to explore the specific regulatory mechanisms of TEA domain family member 1 (TEAD1) in DMED rats. Diabetic ED in rats was induced by streptozotocin and evaluated by the intracavernosal pressure response to electrical stimulation and the apomorphine test. Corpus cavernosum smooth muscle cells (CCSMCs) were isolated to explore the role of TEAD1 in phenotypic transformation. TEAD1 knockdown was performed in DMED rats and CCSMCs using the CRISPR\/Cas9 technology. Hematoxylin\u2013Eosin and Masson staining were applied to observe the penile tissue pathology. The CCSMC function was evaluated by detecting viability and apoptosis. Protein and gene expression levels were examined by Western blot and real\u2010time quantitative polymerase chain reaction. The TEAD1 gene knockdown ameliorated erectile function in DMED rats. Knockdown of TEAD1 in CCSMCs of DMED rats enhanced their viability and inhibited their apoptosis, promoting the transformation of CCSMCs from a synthetic to a contractile phenotype. Furthermore, bioinformatics analysis identified the calcium signaling pathway as a candidate pathway warranting further experimental investigation. TEAD1 knockdown was associated with altered expression of calcium\u2010related proteins and elevated intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    levels. The calcium channel blocker Nimodipine could reverse the improvement of erectile function in DMED rats and the transformation of CCSMC phenotype caused by TEAD1 knockdown. TEAD1 knockdown promotes the transformation of CCSMCs from a synthetic to a contractile phenotype and is associated with modulation of calcium signaling\u2010mediated contractile machinery, contributing to the relief of DMED.\n                  <\/jats:p>","DOI":"10.1002\/ddr.70365","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:42:13Z","timestamp":1786977733000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["TEAD1 Knockdown Ameliorates Diabetic Erectile Dysfunction in Rats and Is Associated With Modulation of Calcium Signaling\u2010Mediated Contractile Machinery"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1687-1367","authenticated-orcid":false,"given":"Tao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Urology, Guangzhou Red Cross Hospital Jinan University Guangzhou China"},{"name":"Department of Urology, Guangzhou Red Cross Hospital Guangdong Medical University Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxiao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Urology, The Second School of Clinical Medicine Southern Medical University, Guangdong Second Provincial General Hospital Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youlong","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Urology, Guangzhou Red Cross Hospital Guangdong Medical University Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weili","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaru","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology, Guangzhou Red Cross Hospital Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology, Guangzhou Red Cross Hospital Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00502.2020"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.17272"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14817"},{"key":"e_1_2_12_5_1","first-page":"870","article-title":"Phosphodiesterase\u20105 Inhibitors as Therapeutics for Cardiovascular Diseases: A Brief Review","volume":"52","author":"Corbic M.","year":"2023","journal-title":"Iranian Journal of Public Health"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2024.1368079"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.01120"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336950.2019.1688910"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.956173"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471141755.ph0547s70"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.575626"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.733420"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27527"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11061595"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39009-z"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.17738"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44318-024-00085-6"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33841-5"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)68237-7"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2016.07.028"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11050997"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1063"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114674"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0306926"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.15504"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.515817"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.93343"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.13438"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/postgradmedj-2016-134073"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.314187"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsxm.2017.08.006"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00354.2023"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-040122-034745"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2023.110846"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.895044"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-0850-6518"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.118.311830"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)03024-3"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8071017"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/dom.15491"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2022.170765"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.3"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1474-8"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1325-7"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1168744"},{"key":"e_1_2_12_46_1","first-page":"927","article-title":"[Henotypic Modulation of Corporal Cavernosum Smooth Muscle Cells and Erectile Dysfunction: Advances in Studies]","volume":"24","author":"Zhao F.","year":"2018","journal-title":"Zhonghua Nan Ke Xue = National Journal of Andrology"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2016.02.021"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.182817"}],"container-title":["Drug Development Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ddr.70365","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ddr.70365","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ddr.70365","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:09:37Z","timestamp":1789024177000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/ddr.70365"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/ddr.70365"],"URL":"https:\/\/doi.org\/10.1002\/ddr.70365","archive":["Portico"],"relation":{},"ISSN":["0272-4391","1098-2299"],"issn-type":[{"value":"0272-4391","type":"print"},{"value":"1098-2299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]},"assertion":[{"value":"2026-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70365"}}