{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:06:13Z","timestamp":1789535173040,"version":"build-2803163510"},"reference-count":71,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mosquito\u2010borne arboviruses, including dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV), represent a growing global health burden, yet no specific antiviral therapies are currently available for most of these infections. This unmet need highlights the importance of identifying novel antiviral compounds from biologically relevant sources such as plant\u2010derived natural products. In this study, a bioactivity\u2010guided approach was applied to methanolic extracts from eight Solanaceae species to identify compounds with antiviral activity against DENV\u20102 in Vero cells. Five extracts significantly reduced viral infectivity, with\n                    <jats:italic>Solanum ovalifolium<\/jats:italic>\n                    and\n                    <jats:italic>Cestrum<\/jats:italic>\n                    sp. achieving complete inhibition at non\u2010cytotoxic concentrations. Subsequent fractionation and purification led to the isolation of four spirostane\u2010type steroidal saponins, structurally characterized by UPLC\u2010DAD\u2010MS and 1D\/2D NMR spectroscopy. Functional assays demonstrated that these compounds inhibit DENV replication, as evidenced by marked reductions in viral RNA levels, NS1 and NS3 protein expression, and infectious viral titers. Stage\u2010specific analyses revealed that their antiviral activity is primarily associated with intracellular phases of the viral life cycle, consistent with inhibition of viral replication and\/or protein processing. Molecular docking analyses supported these findings by predicting favorable interactions with key viral enzymes, particularly NS5 RNA\u2010dependent RNA polymerase and NS3 helicase\/protease. Importantly, the isolated compounds retained antiviral activity against ZIKV and CHIKV, indicating cross\u2010arboviral efficacy. Together, these findings identify spirostane saponins as promising antiviral scaffolds and support their further development as broad\u2010spectrum antiviral agents.\n                  <\/jats:p>","DOI":"10.1002\/ddr.70379","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:34:33Z","timestamp":1789479273000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Solanaceae\u2010Derived Spirostane Saponins Inhibit Dengue Virus Replication and Viral Protein Expression and Exhibit Activity Against Multiple Arboviruses"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3737-9427","authenticated-orcid":false,"given":"Erika Vanessa","family":"Jimenez\u2010Posada","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Ciencias Animales\u2010GRICA, Facultad de Medicina Veterinaria y Zootecnia Universidad Cooperativa de Colombia Bucaramanga Colombia"},{"name":"Grupo de Investigaci\u00f3n Biotecnolog\u00eda\u2010Productos Naturales, Escuela de Qu\u00edmica, Facultad de Tecnolog\u00eda Universidad Tecnol\u00f3gica de Pereira Pereira Colombia"},{"name":"PECET\u2010Facultad de Medicina Universidad de Antioquia Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2998-0842","authenticated-orcid":false,"given":"Oscar Marino","family":"Mosquera\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Biotecnolog\u00eda\u2010Productos Naturales, Escuela de Qu\u00edmica, Facultad de Tecnolog\u00eda Universidad Tecnol\u00f3gica de Pereira Pereira Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2752-4931","authenticated-orcid":false,"given":"Sara M.","family":"Robledo","sequence":"additional","affiliation":[{"name":"PECET\u2010Facultad de Medicina Universidad de Antioquia Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3575-1253","authenticated-orcid":false,"given":"Diego","family":"Durango","sequence":"additional","affiliation":[{"name":"Grupo de Qu\u00edmica de los Productos Naturales y los Alimentos, Departamento de Qu\u00edmica, Facultad de Ciencias Universidad Nacional de Colombia\u2010Sede Medell\u00edn Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3088-1462","authenticated-orcid":false,"given":"Wiston","family":"Qui\u00f1ones","sequence":"additional","affiliation":[{"name":"Qu\u00edmica Org\u00e1nica de Productos Naturales Universidad de Antioquia Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8504-7999","authenticated-orcid":false,"given":"Samuel","family":"Vizca\u00edno\u2010P\u00e1ez","sequence":"additional","affiliation":[{"name":"Qu\u00edmica Org\u00e1nica de Productos Naturales Universidad de Antioquia Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9429-0058","authenticated-orcid":false,"given":"Marlen","family":"Martinez\u2010Gutierrez","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Ciencias Animales\u2010GRICA, Facultad de Medicina Veterinaria y Zootecnia Universidad Cooperativa de Colombia Bucaramanga Colombia"},{"name":"Grupo de Investigaci\u00f3n en Microbiolog\u00eda B\u00e1sica y Aplicada\u2010MICROBA Universidad de Antioquia Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1545","article-title":"Therapeutic Potential of Solanum Surattense Against Hepatitis C Virus in Liver Hepatoma Cells","volume":"37","author":"Aamir M.","year":"2024","journal-title":"Pakistan Journal of Pharmaceutical Sciences"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/S-2001-14332\/ID\/5\/BIB"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/v17020212"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01130-20"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(01)00417-4"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.STEROIDS.2021.108933"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.70108"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.22517\/25395203.8247"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300406n"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ANTIVIRAL.2014.09.007"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/NP50047A033"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2025.115613"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/S10822-023-00522-4\/FIGURES\/8"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.PHYMED.2016.08.001"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2np00003b"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431X2020e10240"},{"key":"e_1_2_9_18_1","first-page":"500","article-title":"Arboviral Diseases Branch, National Center for Emerging and Zoonotic Infectious Diseases, C. Notes From the Field: Chikungunya Virus Spreads in the Americas \u2010 Caribbean and South America, 2013\u20102014","volume":"63","author":"Fischer M.","year":"2014","journal-title":"MMWR. Morbidity and Mortality Weekly Report"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp5074939"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-101416-041848"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/NP0305019\/ASSET\/IMAGES\/LARGE\/NP0305019F1.JPEG"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ONEHLT.2017.06.001"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0042-118387"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-S13-S22"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/d4md00894d"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-26\/FIGURES\/4"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PPAT.1005451"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26113430"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens14010063"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(11)60015-0"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15020348"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2019.05.004"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-021-03386-z"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/PLANTS10071280"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.VIRUSRES.2007.09.008"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.21608\/EJCHEM.2021.71934.3581"},{"key":"e_1_2_9_37_1","unstructured":"National Academies of Sciences Eg and Medicine. Division HaM Health BoG et al. Mitigating Arboviral Threat and Strengthening Public Health Preparedness: Proceedings of a Workshop.2024."},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880200500509124"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/164878"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.21315\/MJMS2018.25.5.2"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/FCIMB.2023.1061937\/BIBTEX"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44298-025-00112-1"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1076\/PHBI.41.2.107.14240"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0714-0"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines12010087"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.01.036"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijregi.2024.100360"},{"key":"e_1_2_9_48_1","unstructured":"Ram\u00edrez C\u00e1rdenas A. G.Isaza Mej\u00eda andJ. E.P\u00e9rez C\u00e1rdenasEspecies vegetales investigadas por sus propiedades antimicrobianas inmunomoduladoras e hipoglicemiantes en el departamento de Caldas (Colombia Sudam\u00e9rica). Biosalud.2013.https:\/\/revistasojs.ucaldas.edu.co\/index.php\/biosalud\/article\/view\/4702013 p. 59\u201082."},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00336"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/MOLECULES29061316\/S1"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.SYNRES.2014.07.010"},{"key":"e_1_2_9_52_1","doi-asserted-by":"crossref","unstructured":"Rocha D. A.\u2010O. T.Sisnande D. A.\u2010O.Gavino\u2010Leopoldino et al. Antiviral Cytoprotective and Anti\u2010Inflammatory Effect of Ampelozizyphus amazonicus Ducke Ethanolic Wood Extract on Chikungunya Virus Infection. LID \u2010https:\/\/doi.org\/10.3390\/v15112232[doi] LID \u2212 2232.","DOI":"10.3390\/v15112232"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0126360"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/v9040091"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-025-06314-5"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/np9705165"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/CMDC.200700010"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.607341"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules19068151"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-024-03034-6"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30030482"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-019-2695-1"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.2174\/2666958702101010235"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1515\/ZNC-2009-11-1210\/MACHINEREADABLECITATION\/RIS"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1515\/ZNC-2009-11-1210"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2020.1788022"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30518-7"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.16.10278-10288.2005"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02283-06"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1073\/PNAS.1620161114"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2025.2463006"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26010224"}],"container-title":["Drug Development Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ddr.70379","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ddr.70379","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ddr.70379","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T04:58:18Z","timestamp":1789534698000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/ddr.70379"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":71,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1002\/ddr.70379"],"URL":"https:\/\/doi.org\/10.1002\/ddr.70379","archive":["Portico"],"relation":{},"ISSN":["0272-4391","1098-2299"],"issn-type":[{"value":"0272-4391","type":"print"},{"value":"1098-2299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"2026-04-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70379"}}