{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T16:59:44Z","timestamp":1781024384749,"version":"3.54.1"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T00:00:00Z","timestamp":1670544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Psychobiology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, based on the excitatory\/inhibitory imbalance theory of autism, the time window of GABA switch, the role of K\u2010Cl co\u2010transporter 2 (KCC2) in adjustment GABA switch, and brain permeability to erythropoietin (EPO), the effects of postnatal \u2010EPO and\u2010 nano\u2010 erythropoietin (NEPO) have been evaluated in the valproic acid (VPA) rat model of autism. The VPA was administered for animal modeling of autism at gestational day (GD) 12.5 (600 mg\/kg). Male offsprings were injected with EPO and NEPO in a clinically proper postnatal dosing regimen on postnatal days (PND) 1\u20135, and autistic\u2010like behaviors were tested at the end of the first month. Then animals were sacrificed, and neuron morphology and KCC2 expression were examined by Nissl staining and Western blot. According to our findings, high\u2010dose NEPO improved autism\u2010associated phenotypes. Neuroprotective effects of EPO and NEPO have been shown in the hippocampus. Postnatal NEPO treatment reversed KCC2 expression abnormalities induced by prenatal VPA. Our results might support the role of KCC2 in ASD and the excitatory\/inhibitory imbalance hypothesis. We suggested Nano\u2010 erythropoietin and other KCC2 interventions as a new approach to the early treatment and prevention of autism.<\/jats:p>","DOI":"10.1002\/dev.22353","type":"journal-article","created":{"date-parts":[[2022,12,10]],"date-time":"2022-12-10T06:44:58Z","timestamp":1670654698000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The effects of postnatal erythropoietin and nano\u2010erythropoietin on behavioral alterations by mediating K\u2010Cl co\u2010transporter 2 in the valproic acid\u2010induced rat model of autism"],"prefix":"10.1002","volume":"65","author":[{"given":"Sara","family":"Haratizadeh","sequence":"first","affiliation":[{"name":"Student Research Committee Kerman University of Medical Sciences  Kerman Iran"},{"name":"Department of Anatomical Sciences, Afzalipour School of Medicine Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Ranjbar","sequence":"additional","affiliation":[{"name":"Pharmaceutics Research Center, Institute of Neuropharmacology Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Darvishzadeh\u2010Mahani","sequence":"additional","affiliation":[{"name":"Neuroscience Research Center, Institute of Neuropharmacology Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Basiri","sequence":"additional","affiliation":[{"name":"Neuroscience Research Center, Institute of Neuropharmacology Kerman University of Medical Sciences  Kerman Iran"},{"name":"Department of Anatomical Sciences, Afzalipour School of Medicine Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoumeh","family":"Nozari","sequence":"additional","affiliation":[{"name":"Neuroscience Research Center, Institute of Neuropharmacology Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15569543.2020.1747495"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1073858412438697"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13229-018-0222-8"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2014.00070"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/61044"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.111.637090"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2019.01.027"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nlm.2019.107082"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddv273"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jdn.10005"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068851"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2014.10.003"},{"issue":"2019","key":"e_1_2_9_14_1","first-page":"115","article-title":"Early enriched environment prevents cognitive impairment in an animal model of schizophrenia induced by MK\u2010801: Role of hippocampal BDNF","volume":"1711","author":"Faatehi M.","year":"2020","journal-title":"Brain Res"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10741-008-9083-0"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-180668"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jdn.10096"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.09.017"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/9247493"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2014.06.009"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1515\/tnsci-2020-0008"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3171\/FOC\/2008\/25\/9\/E22"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3819"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.100364"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2016.05.011"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2020.152500"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.03.013"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2017.06.018"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-019-05357-2"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370214523703"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2007.03.001"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2014.05.001"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2014.11.004"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2021.103062"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2020.102073"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1524013113"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247190"}],"container-title":["Developmental Psychobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dev.22353","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dev.22353","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dev.22353","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T09:35:27Z","timestamp":1692524127000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dev.22353"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,9]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1002\/dev.22353"],"URL":"https:\/\/doi.org\/10.1002\/dev.22353","archive":["Portico"],"relation":{},"ISSN":["0012-1630","1098-2302"],"issn-type":[{"value":"0012-1630","type":"print"},{"value":"1098-2302","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,9]]},"assertion":[{"value":"2022-08-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22353"}}