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The risk of developing mental illnesses in adulthood is increased by exposure to stress in early periods of life. The available data indicate that puberty is especially characterized by the development of the neural system and emotionality and is a \u201cstress\u2010sensitive\u201d period. The presented study assessed the short\u2010term changes in the expression of RLN\u20103 and RXFP\u20103 mRNA in the stress\u2010dependent brain regions in male pubertal Wistar rats that had been subjected to acute stress. Three stressors were applied from 42 to 44 postnatal days (first day: a single forced swim; second day: stress on an elevated platform that was repeated three times; third day: restraint stress three times). Anxiety (open field, elevated plus maze test) and anhedonic\u2010like behavior (sucrose preference test) were estimated during these tests. The corticosterone (CORT) levels and blood morphology were estimated. We found that the RXFP\u20103 mRNA expression decreased in the brainstem, whereas it increased in the hypothalamus 72\u00a0h after acute stress. These molecular changes were accompanied by the increased levels of CORT and anxiety\u2010like behavior detected in the open field test that had been conducted earlier, that is, 24\u00a0h after the stress procedure. These findings shed new light on the neurochemical changes that are involved in the compensatory response to adverse events in pubertal male rats and support other data that suggest a regulatory interplay between the RLN\u20103 pathway and the hypothalamus\u2013pituitary\u2013adrenal axis activity in the mechanisms of anxiety\u2010like behavior.<\/jats:p>","DOI":"10.1002\/dev.22523","type":"journal-article","created":{"date-parts":[[2024,7,6]],"date-time":"2024-07-06T05:15:06Z","timestamp":1720242906000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Acute Stress Affects the Relaxin\/Insulin\u2010Like Family Peptide Receptor 3 mRNA Expression in Brain of Pubertal Male Wistar Rats"],"prefix":"10.1002","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5860-9885","authenticated-orcid":false,"given":"Mi\u0142osz","family":"Go\u0142yszny","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Faculty of Medical Sciences in Katowice Medical University of Silesia  Katowice Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2775-4887","authenticated-orcid":false,"given":"Micha\u0142","family":"Zieli\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medical Sciences in Katowice Medical University of Silesia  Katowice Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Obuchowicz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medical Sciences in Katowice Medical University of Silesia  Katowice Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1461145704004808"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2009.06.019"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166\u20104328(01)00180\u20102"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2014.11.012"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2007.11.069"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109384(91)90546\u2010Z"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304\u20103940(92)90400\u20102"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091\u20103057(94)00419\u2010J"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2010.09.032"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109384(82)90072\u20105"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10253890.2018.1470238"},{"issue":"5","key":"e_1_2_10_13_1","first-page":"409","article-title":"An \u201cOld\u201d and \u201cNew\u201d Neuropeptides as Modulators of the Stress Axis (Hypothalamus\u2013Pituitary\u2013Adrenal)","volume":"2","author":"Go\u0142yszny M.","year":"2018","journal-title":"Psychiatria Varia Medica"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2019.02.002"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2021.102216"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2022.02.005"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3934\/Neuroscience.2022022"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1303"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-313-4_16"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0376\u20106357(93)90026\u2010N"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2003.07.001"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168\u20108278(94)80099\u20105"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2013.10.004"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/10253890.2011.634036"},{"key":"e_1_2_10_25_1","volume-title":"Behavioral Models in Stress Research","author":"Kalueff A. 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