{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T07:17:34Z","timestamp":1781767054034,"version":"3.54.5"},"reference-count":18,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,9,18]],"date-time":"2009-09-18T00:00:00Z","timestamp":1253232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diabetes Metabolism Res"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Older studies of diabetes development typically utilized a 7\u2010day incubation polyethylene glycol competitive insulin autoantibody assay (CIAA). Our standard micro\u2010IAA assay (mIAA) utilizes precipitation with proteins A\/G and 1\u2010day incubation (1\u2010day mIAA), but is less sensitive compared to the CIAA assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed CIAA and mIAA assays in various conditions. We analyzed serum samples from 446 type 1 diabetes patients, from another set of 247 type 1 diabetes patients within 2 weeks of initiation of insulin treatment, from 150 healthy control donors, from 22 healthy participants in the diabetes autoimmunity study in the young (DAISY), and also coded sera from 50 patients with newly diagnosed type 1 diabetes and 50 blood donor control samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the process of our study, we found that the key condition was the incubation time. Therefore, we extended the incubation time to 7 days (7\u2010day mIAA assay). No CIAA\u2010negative control was positive with either 1\u2010day or 7\u2010day mIAA. In a new onset type 1 diabetes and at risk cohorts (DAISY study), the 7\u2010day mIAA identified an additional 18% as being positive along with 16% of those who were initially 1\u2010day mIAA negative and CIAA positive. Most subjects detectable only with the 7\u2010day mIAA assay had intermediate levels of CIAA (80\u2013300 nU\/mL) (<jats:italic>p<\/jats:italic> = 0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The 7\u2010day mIAA assay identifies a small but significant additional subset of individuals positive on the CIAA assay, while preserving specificity. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dmrr.1014","type":"journal-article","created":{"date-parts":[[2009,9,18]],"date-time":"2009-09-18T14:58:18Z","timestamp":1253285898000},"page":"665-670","source":"Crossref","is-referenced-by-count":23,"title":["Comparison of insulin autoantibody: polyethylene glycol and micro\u2010IAA 1\u2010day and 7\u2010day assays"],"prefix":"10.1002","volume":"25","author":[{"given":"Naru","family":"Babaya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian","family":"Rewers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Nakayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer M.","family":"Barker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George S.","family":"Eisenbarth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,9,18]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.8.3912"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.3.882"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-031887"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2005.09.002"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1361.061"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00290492"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.28.5.1068"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1997.0154"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.040556697"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.5.1128"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.36.11.1286"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03523"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03625"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.45.7.926"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.47.12.1857"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.554"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200421307"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.42.8.1202"}],"container-title":["Diabetes\/Metabolism Research and Reviews"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdmrr.1014","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dmrr.1014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T20:23:33Z","timestamp":1696969413000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dmrr.1014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,18]]},"references-count":18,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["10.1002\/dmrr.1014"],"URL":"https:\/\/doi.org\/10.1002\/dmrr.1014","archive":["Portico"],"relation":{},"ISSN":["1520-7552","1520-7560"],"issn-type":[{"value":"1520-7552","type":"print"},{"value":"1520-7560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,18]]}}}