{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:41:37Z","timestamp":1786977697919,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,11,3]],"date-time":"2010-11-03T00:00:00Z","timestamp":1288742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diabetes Metabolism Res"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      The mechanism by which islet amyloid polypeptide (IAPP) inhibits insulin release is unclear. We hypothesized that reduced voltage\u2010gated calcium channel activity and intracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      concentration might contribute to IAPP\u2010mediated inhibition of glucose\u2010stimulated insulin release.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Research design and methods<\/jats:title>\n                    <jats:p>\n                      Rat islet beta cells were cultured and treated with various extracellular concentrations of IAPP, and insulin release was stimulated via addition of glucose. Activation voltage, high voltage\u2010gated calcium channel currents, intracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      concentration, and insulin secretion were detected by patch clamp electrophysiology, fluorescent digital imaging microscopy using calcium\u2010sensitive fluorescent dye, and radioimmunoassay, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      High voltage\u2010gated calcium channel currents, intracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      concentration, and insulin secretion increased in a dose\u2010dependent manner when rat beta cells were exposed to glucose. After short\u2010term IAPP treatment (5 or 10 \u00b5M), these parameters decreased significantly in glucose\u2010stimulated beta cells. However, no significant changes were observed with lower doses of IAPP.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Glucose\u2010stimulated islet beta\u2010cell high voltage\u2010gated calcium channels were activated in conjunction with insulin secretion, while high extracellular concentrations of IAPP inhibited beta\u2010cell high voltage\u2010gated calcium channel activation and insulin secretion in a dose\u2010dependent manner. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dmrr.1140","type":"journal-article","created":{"date-parts":[[2010,11,5]],"date-time":"2010-11-05T19:22:06Z","timestamp":1288984926000},"page":"28-34","source":"Crossref","is-referenced-by-count":18,"title":["Islet amyloid polypeptide acts on glucose\u2010 stimulated beta cells to reduce voltage\u2010gated calcium channel activation, intracellular Ca\n                    <sup>2+<\/sup>\n                    concentration, and insulin secretion"],"prefix":"10.1002","volume":"27","author":[{"given":"Tiehong","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinxia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingjun","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Zang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,11,3]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.11.3881"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)90825-7"},{"key":"e_1_2_6_4_2","first-page":"291","article-title":"The pancreatic islet cells in insular amyloidosis in human diabetic and non\u2010diabetic adults","volume":"81","author":"Westermark P","year":"1973","journal-title":"Acta Pathol Microbiol Scand A"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00452067"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090532"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-8227(92)90062-V"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0405"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/368756a0"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.14.7283"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-8227(92)90073-Z"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)91325-7"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006676-200104000-00013"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.42.10.1514"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.53.6.1509"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1006\/cbir.2000.0643"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)92529-1"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.2337\/db07-0991"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1155\/2008\/421287"},{"issue":"6","key":"e_1_2_6_20_2","doi-asserted-by":"crossref","first-page":"662","DOI":"10.2337\/diab.41.6.662","article-title":"Stimulus\u2010secretion coupling in beta\u2010cells of transplantable human islets of Langerhans. 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