{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:39:50Z","timestamp":1786977590919,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,5,11]],"date-time":"2012-05-11T00:00:00Z","timestamp":1336694400000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diabetes Metabolism Res"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Mammalian excision repair cross\u2010complementing 1 (ERCC1) and ERCC4 (a.k.a xeroderma pigmentosum complementation group F) are nucleotide excision repair enzymes, which excise the 5\u2032 end of damaged DNA. ERCC1 and ERCC4 have an interactive relationship with poly (adenosine diphosphate ribose) polymerase (PARP). We studied the role of ERCC1 and ERCC4 in glucose\u2010induced extracellular matrix protein production in human endothelial cells and in the retinas and kidneys of streptozotocin diabetic rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Human umbilical vein endothelial cells were grown with low (5\u2009mM) and high glucose (25\u2009mM). The cells were subjected to ERCC1 and ERCC4 small interfering RNA transfections, PARP blocker (3\u2010aminobenzamide, ABA) and p300 blocker (curcumin). Retinas and kidneys from 1\u2010month\u2010old streptozotocin diabetic rats with or without treatment with curcumin and ABA were examined. Cells and tissues were studied for oxidative stress markers, fibronectin, ERCC1 and ERCC4, PARP and p300 mRNA. Western blot of nuclear proteins was performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>ERCC1 and ERCC4 messenger RNA and protein levels were higher in high glucose than in low glucose, along with increasing oxidative stress and augmented p300 and fibronectin production. ABA, curcumin, ERCC1 and ERCC4 silencing reduced such upregulations and oxidative stress. Similar changes were seen in the kidneys and retinas of diabetic rats. ABA and curcumin treatment significantly reduced such changes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These data indicate that glucose\u2010induced ERCC1 and ERCC4 upregulation leads to increased fibronectin production via a p300\u2010dependent pathway in umbilical endothelial cells, as well as in the retina and in the kidneys of streptozotocin diabetic rats. ERCC1 and ERCC4 may play important roles in the development of diabetic retinopathy and nephropathy. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dmrr.2279","type":"journal-article","created":{"date-parts":[[2012,1,6]],"date-time":"2012-01-06T16:27:14Z","timestamp":1325867234000},"page":"329-337","source":"Crossref","is-referenced-by-count":25,"title":["Genotoxic stress and activation of novel DNA repair enzymes in human endothelial cells and in the retinas and kidneys of streptozotocin diabetic rats"],"prefix":"10.1002","volume":"28","author":[{"given":"Chunyan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology, Schulich School of Medicine and Dentistry University of Western Ontario  London ON Canada"},{"name":"School of Public Health Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biju","family":"George","sequence":"additional","affiliation":[{"name":"Department of Pathology, Schulich School of Medicine and Dentistry University of Western Ontario  London ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shali","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Schulich School of Medicine and Dentistry University of Western Ontario  London ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Pathology, Schulich School of Medicine and Dentistry University of Western Ontario  London ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaokun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology, Norman Bethune College of Medicine Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subrata","family":"Chakrabarti","sequence":"additional","affiliation":[{"name":"Department of Pathology, Schulich School of Medicine and Dentistry University of 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