{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:10:03Z","timestamp":1787508603989,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,3,8]],"date-time":"2002-03-08T00:00:00Z","timestamp":1015545600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diabetes Metabolism Res"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Amylin is a peptide co\u2010secreted with insulin by pancreatic \u03b2\u2010cells. A role for amylin in the pathogenesis of type 2 diabetes mellitus (DM2) has been suggested by\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      studies indicating an effect of amylin to cause insulin resistance and\/or inhibit insulin secretion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We have determined the effect of endogenous amylin on insulin secretion and insulin action in humans by performing 4\u2010h hyperglycemic clamps during infusion of placebo or a specific amylin receptor antagonist (ARA) in paired, double\u2010blinded, crossover studies. We studied nine healthy lean, ten healthy obese (BMI&gt;27) and ten obesity\u2010matched DM2 subjects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Infusion of ARA alone had no effect on basal insulin, glucose or glucose turnover in any group. Under combined hyperglycemia and ARA infusion, lean subjects displayed a 32% augmentation in insulin levels [AUC 33\u2009565\u00b13556 (placebo) to 44\u2009562\u00b11379 (ARA)\u2009pmol\/l\/min,\n                      <jats:italic>p<\/jats:italic>\n                      &lt;0.01]. The concomitant increase in glucose disposal rate (GDR) was proportionate, indicating no change in insulin sensitivity (ISI 27.7\u00b12.7 vs 27.3\u00b12.1,\n                      <jats:italic>p<\/jats:italic>\n                      =NS). In obese subjects, basal insulin and the rise in insulin during the clamp were greater (AUC I 44% increase from 82\u2009054\u00b115\u2009407 to 117\u2009922\u00b127\u2009085,\n                      <jats:italic>p<\/jats:italic>\n                      &lt;0.01), and also accompanied by a proportionate rise in GDR reflecting an unchanged insulin sensitivity (ISI 12.1\u00b12.9 vs 10.8\u00b13.0,\n                      <jats:italic>p<\/jats:italic>\n                      =NS). In lean and obese subjects, the C\u2010peptide response to hyperglycemia was also augmented by ARA (\n                      <jats:italic>p<\/jats:italic>\n                      =0.007). No effect of ARA on insulin secretion or action was observed in diabetic subjects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The present data are consistent with an effect of endogenous amylin on the \u03b2\u2010cell to modulate and\/or restrain insulin secretion, and indicate that endogenous amylin does not affect insulin action. These observations provide the first human evidence that amylin plays a role in the modulation of insulin secretion. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dmrr.263","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T19:45:33Z","timestamp":1030304733000},"page":"118-126","source":"Crossref","is-referenced-by-count":36,"title":["Role of amylin in insulin secretion and action in humans: antagonist studies across the spectrum of insulin sensitivity"],"prefix":"10.1002","volume":"18","author":[{"given":"Kieren 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