{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T10:53:24Z","timestamp":1762340004061},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,5,12]],"date-time":"2005-05-12T00:00:00Z","timestamp":1115856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diabetes Metabolism Res"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Pericyte ghosts and acellular capillaries are well known as early histological changes resulting from diabetic retinopathy. These histological changes mean that the cell death of retinal microvessels has accelerated. It was reported that apoptosis of retinal microvascular cells (RMCs) was increased in diabetic patients. Therefore, we investigated apoptosis of RMCs in Goto\u2010Kakizaki (GK) rats, a type 2 diabetic model, and involvement with antioxidants (a combination of vitamins C and E) or a novel inhibitor of advanced glycation, OPB\u20109195.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>GK rats were treated with the antioxidants combination or OPB\u20109195 for 36 weeks. We obtained isolated preparations of the vascular network from their retinas by trypsin digestion. Apoptosis of retinal vascular cells was detected with terminal deoxynucleotidyl transferase\u2010mediated dUTP nick end labeling (TUNEL) assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that apoptosis of RMCs was increased in the diabetic GK rats. Furthermore, a combination of vitamins C and E and an advanced glycation end\u2010products inhibitor mostly inhibited this increased apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We concluded that apoptosis of RMCs was a good marker that indicates the progression of diabetic retinopathy in GK rats. Both oxidative stress and the accumulation of advanced glycation end\u2010products appears to promote the apoptosis of retinal microvascular cells, and antioxidants or advanced glycation end\u2010products inhibitors might ameliorate diabetic retinopathy. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dmrr.562","type":"journal-article","created":{"date-parts":[[2005,5,12]],"date-time":"2005-05-12T12:16:36Z","timestamp":1115900196000},"page":"38-45","source":"Crossref","is-referenced-by-count":45,"title":["Antioxidants and an inhibitor of advanced glycation ameliorate death of retinal microvascular cells in diabetic retinopathy"],"prefix":"10.1002","volume":"22","author":[{"given":"Shigeru","family":"Yatoh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Mizutani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomotaka","family":"Yokoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadahiko","family":"Kozawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohito","family":"Sone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Toyoshima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Shimano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Kawakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukichi","family":"Okuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhiro","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,12]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1961.00960010368014"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1968.00980050334007"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118746"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.137.453"},{"issue":"2","key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.2337\/diab.43.2.281","article-title":"Impact of diabetic inheritance on glucose tolerance and insulin secretion in spontaneously diabetic GK\u2010Wistar rats","volume":"43","author":"Abdel Halim SM","year":"1994","journal-title":"Diabetes"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00399086"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.44.11.1280"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02573824"},{"key":"e_1_2_6_10_2","first-page":"26","article-title":"Neurological findings in spontaneously diabetic rats","volume":"581","author":"Goto Y","year":"1982","journal-title":"Excerpta Med Int Cong Ser"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1056-8727(96)00049-9"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)90022-2"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.46.2.S14"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.46.5.895"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001250051198"},{"issue":"9","key":"e_1_2_6_16_2","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1681\/ASN.V1191719","article-title":"Mechanism of the inhibitory effect of OPB\u20109195 [(+\/\u2212)\u20102\u2010isopropylidenehydrazono\u20104\u2010oxo\u2010thiazolidin\u20105\u2010ylacetanilide] on advanced glycation end product and advanced lipoxidation end product formation","volume":"11","author":"Miyata T","year":"2000","journal-title":"J Am Soc Nephrol"},{"issue":"9","key":"e_1_2_6_17_2","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1681\/ASN.V1191744","article-title":"Advanced glycation and lipoxidation end products: role of reactive carbonyl compounds generated during carbohydrate and lipid metabolism","volume":"11","author":"Miyata T","year":"2000","journal-title":"J Am Soc Nephrol"},{"issue":"2","key":"e_1_2_6_18_2","first-page":"600","article-title":"Vascular wall von Willebrand factor in human diabetic retinopathy","volume":"35","author":"Boeri D","year":"1994","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.3.493"},{"issue":"2","key":"e_1_2_6_20_2","first-page":"219","article-title":"Differentiation between cellular apoptosis and necrosis by the combined use of in situ tailing and nick translation techniques","volume":"71","author":"Gold R","year":"1994","journal-title":"Lab Invest"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.3109\/02713689409015087"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)89449-6"},{"issue":"15","key":"e_1_2_6_23_2","doi-asserted-by":"crossref","first-page":"10 211","DOI":"10.1016\/S0021-9258(19)50004-7","article-title":"Immunochemical evidence for the presence of advanced glycation end products in human lens proteins and its positive correlation with aging","volume":"267","author":"Araki N","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116696"},{"issue":"12","key":"e_1_2_6_25_2","first-page":"3972","article-title":"Response of capillary cell death to aminoguanidine predicts the development of retinopathy: comparison of diabetes and galactosemia","volume":"41","author":"Kern TS","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(87)90489-9"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/35008121"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.50.8.1938"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.2.517"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.1999.tb00358.x"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0026-0495(99)90128-2"},{"issue":"3","key":"e_1_2_6_32_2","first-page":"577","article-title":"Effect of aminoguanidine on lipid peroxidation and activities of antioxidant enzymes in the diabetic kidney","volume":"46","author":"Kedziora Kornatowska K","year":"1998","journal-title":"Biochem Mol Biol Int"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.47.7.1114"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9530550"},{"issue":"3","key":"e_1_2_6_35_2","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1177\/089686089901900305","article-title":"Suppression of N(epsilon)\u2010(carboxymethyl)lysine generation by the antioxidant N\u2010acetylcysteine","volume":"19","author":"Nakayama M","year":"1999","journal-title":"Perit Dial Int"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(99)00038-6"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119853"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.22.12964"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.17.9982"}],"container-title":["Diabetes\/Metabolism Research and Reviews"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dmrr.562","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T18:38:24Z","timestamp":1697654304000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dmrr.562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,5,12]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,1]]}},"alternative-id":["10.1002\/dmrr.562"],"URL":"https:\/\/doi.org\/10.1002\/dmrr.562","archive":["Portico"],"relation":{},"ISSN":["1520-7552","1520-7560"],"issn-type":[{"value":"1520-7552","type":"print"},{"value":"1520-7560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,5,12]]}}}