{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T19:40:52Z","timestamp":1783021252797,"version":"3.54.6"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabetes Metabolism Res"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In recent decades, obesity and diabetes have reached pandemic levels, with obesity now recognised as a major health risk factor. Evidence shows that metabolic diseases are more pronounced in the offspring of malnourished parents, suggesting that predisposition can be inherited via epigenetic information in gametes. This has sparked growing interest in small regulatory RNAs in sperm as carriers of epigenetic inheritance. However, the functional annotation of dysregulated sperm microRNAs (miRNAs) in obesity and diabetes remains limited. This work addresses this gap by analysing publicly available datasets of diet\u2010regulated sperm miRNAs and linking them to genes functionally associated with obesity and diabetes. We systematically identified diet\u2010responsive sperm miRNAs and overlapped their predicted targets with genes associated with metabolic phenotypes, as catalogued by the International Mouse Phenotyping Consortium (IMPC). First, in a sequence\u2010based approach, we uncovered 11,272 and 6528 potential target genes for miRNAs regulated by the acute and chronic HFD interventions, respectively. Second, by overlapping these predicted target genes of sperm miRNAs with our IMPC\u2010derived list of 889 genes associated with obesity and diabetes, we identified 805 acute\u2010 and 546 chronic\u2010HFD predicted response genes. This approach thus associates function with regulated miRNAs and revealed distinct miRNA\u2013gene networks in acute versus chronic HFD models, including shared nodes in pathways related to insulin signalling, lipid metabolism, and \u03b2\u2010cell function. To support further research, we provide the field with the\n                    <jats:italic>ShinyFatSperm<\/jats:italic>\n                    App (\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/reproproteomics.shinyapps.io\/ShinyFatSperm\/\">https:\/\/reproproteomics.shinyapps.io\/ShinyFatSperm\/<\/jats:ext-link>\n                    ), which facilitates the functional interpretation of diet\u2010regulated sperm miRNAs and enables users to explore their roles in the intergenerational transmission of metabolic disease risk. Taken together, our findings reinforce the concept that paternal dietary exposures can influence offspring health through epididymal\u2010 and sperm\u2010borne miRNAs, and related epigenetic mechanisms. This work provides a roadmap for hypothesis\u2010driven investigation into the intergenerational inheritance of metabolic diseases and highlights the urgent need for translational strategies to interrupt this cycle.\n                  <\/jats:p>","DOI":"10.1002\/dmrr.70157","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T09:57:57Z","timestamp":1774259877000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Epigenetic Legacy: The Role of Sperm miRNAs in the Paternal Inheritance of Diabetes and Obesity Development"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8348-8650","authenticated-orcid":false,"given":"Katharina","family":"Laurent","sequence":"first","affiliation":[{"name":"Helmholtz Zentrum M\u00fcnchen, German Research Center for Environmental Health Institute of Experimental Genetics  Neuherberg Germany"},{"name":"German Center for Diabetes Research (DZD)  Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8815-1409","authenticated-orcid":false,"given":"Raffaele","family":"Teperino","sequence":"additional","affiliation":[{"name":"Helmholtz Zentrum M\u00fcnchen, German Research Center for Environmental Health Institute of Experimental Genetics  Neuherberg Germany"},{"name":"German Center for Diabetes Research (DZD)  Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7898-2353","authenticated-orcid":false,"given":"Martin","family":"Hrab\u011b de Angelis","sequence":"additional","affiliation":[{"name":"Helmholtz Zentrum M\u00fcnchen, German Research Center for Environmental Health Institute of Experimental Genetics  Neuherberg Germany"},{"name":"German Center for Diabetes Research (DZD)  Neuherberg Germany"},{"name":"Helmholtz Zentrum M\u00fcnchen, German Research Center for Environmental Health (GmbH) German Mouse Clinic  Neuherberg Germany"},{"name":"Chair of Experimental Genetics, TUM School of Life Sciences Technische Universit\u00e4t M\u00fcnchen  Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1804-1826","authenticated-orcid":false,"given":"David A.","family":"Skerrett\u2010Byrne","sequence":"additional","affiliation":[{"name":"Helmholtz Zentrum M\u00fcnchen, German Research Center for Environmental Health Institute of Experimental Genetics  Neuherberg Germany"},{"name":"German Center for Diabetes Research (DZD)  Neuherberg Germany"},{"name":"Reproductive and Family Health Program Hunter Medical Research Institute  Awabakal Country, Newcastle New South Wales Australia"},{"name":"School of Biomedical Sciences and Pharmacy, College of Health, Medicine and Wellbeing The University of Newcastle  Awabakal Country, Callaghan New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7874-3822","authenticated-orcid":false,"given":"Johannes","family":"Beckers","sequence":"additional","affiliation":[{"name":"Helmholtz Zentrum M\u00fcnchen, German Research Center for Environmental Health Institute of Experimental Genetics  Neuherberg Germany"},{"name":"German Center for Diabetes Research (DZD)  Neuherberg Germany"},{"name":"Chair of Experimental Genetics, TUM School of Life Sciences Technische Universit\u00e4t M\u00fcnchen  Freising Germany"},{"name":"INFRAFRONTIER ERIC  Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2022.155217"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2023.1171507"},{"key":"e_1_2_9_4_1","unstructured":"IDF Diabetes 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