{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:56:48Z","timestamp":1791421008426,"version":"4.3.3"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,12,27]],"date-time":"2007-12-27T00:00:00Z","timestamp":1198713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2008,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The adult zebrafish retina possesses a robust regenerative response. In the light\u2010damaged retina, M\u00fcller glial cell divisions precede regeneration of rod and cone photoreceptors. Neuronal progenitors, which arise from the M\u00fcller glia, continue to divide and use the M\u00fcller glial cell processes to migrate to the outer nuclear layer and replace the lost photoreceptors. We tested the necessity of M\u00fcller glial cell division for photoreceptor regeneration. As knockdown tools were unavailable for use in the adult zebrafish retina, we developed a method to conditionally inhibit the expression of specific proteins by\n                    <jats:italic>in vivo<\/jats:italic>\n                    electroporation of morpholinos. We determined that two separate morpholinos targeted against the proliferating cell nuclear antigen (PCNA) mRNA reduced PCNA protein levels. Furthermore, injection and\n                    <jats:italic>in vivo<\/jats:italic>\n                    electroporation of PCNA morpholinos immediately prior to starting intense light exposure inhibited both M\u00fcller glial cell proliferation and neuronal progenitor marker Pax6 expression. PCNA knockdown additionally resulted in decreased expression of glutamine synthetase in M\u00fcller glia and M\u00fcller glial cell death, while amacrine and ganglion cells were unaffected. Finally, histological and immunological methods showed that long\u2010term effects of PCNA knockdown resulted in decreased numbers of M\u00fcller glia and the failure to regenerate rod photoreceptors, short single cones, and long single cones. These data suggest that M\u00fcller glial cell division is necessary for proper photoreceptor regeneration in the light\u2010damaged zebrafish retina and are consistent with the M\u00fcller glia serving as the source of neuronal progenitor cells in regenerating teleost retinas. \u00a9 2007 Wiley Periodicals, Inc. Develop Neurobiol, 2008.\n                  <\/jats:p>","DOI":"10.1002\/dneu.20596","type":"journal-article","created":{"date-parts":[[2007,12,27]],"date-time":"2007-12-27T15:16:25Z","timestamp":1198768585000},"page":"392-408","source":"Crossref","is-referenced-by-count":144,"title":["Inhibition of M\u00fcller glial cell division blocks regeneration of the light\u2010damaged zebrafish retina"],"prefix":"10.1002","volume":"68","author":[{"given":"Ryan","family":"Thummel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean C.","family":"Kassen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob E.","family":"Montgomery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer M.","family":"Enright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David 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