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In the present study, using black porgy, <jats:italic>Acanthopagrus schlegeli<\/jats:italic>, as primary experimental model, we investigated the temporal expression patterns of receptors for androgen (<jats:italic>ar<\/jats:italic>) and estrogen (<jats:italic>esr1<\/jats:italic> and <jats:italic>esr2a<\/jats:italic>) in the brain during posthatching ages and analyzed them against the timing of gonadal germ cell development. We hypothesized that endogenous estrogens naturally masculinize the brain of black porgy. The expression of sex steroid receptors was studied in relation to a wider suite of other related genes (<jats:italic>nr5a2, nr0b1, star<\/jats:italic>, and <jats:italic>cyp19a1b<\/jats:italic>) to provide some insight into the monomale sex differentiation pattern observed in this species. Our results revealed a highly significant increase in <jats:italic>esr1<\/jats:italic> together with the increase in <jats:italic>esr2a<\/jats:italic> at 120 dph (days posthatching), suggesting a significant role for <jats:italic>esr<\/jats:italic> in sex differentiation in this species. Temporal expression patterns of <jats:italic>nr5a2<\/jats:italic>, <jats:italic>nr0b1<\/jats:italic>, <jats:italic>star<\/jats:italic>, sex steroid receptors, and <jats:italic>cyp19a1b<\/jats:italic> in the brain provided evidence for their physiological roles in the monomale sex differentiation in this species. The expression of <jats:italic>nr5a2<\/jats:italic>, <jats:italic>star, ar, esr1, esr2a<\/jats:italic>, and <jats:italic>cyp19a1b<\/jats:italic> increased at 120 dph, a period when brain sex differentiation probably occurs in this species. The study also suggests that neurosteroidogenesis in black porgy may be regulated by both <jats:italic>nr5a2<\/jats:italic>\u2010dependent and <jats:italic>nr5a2<\/jats:italic>\u2010independent mechanisms. The results demonstrated striking differences in the abundance of the gene transcripts in discrete brain region throughout ontogeny. In addition, the sex steroid hormone levels and aromatase activity in brain at different developmental states and the changes in the gene expression patterns in response to aromatase inhibitor treatment are also discussed. \u00a9 2009 Wiley Periodicals, Inc. Develop Neurobiol, 2009<\/jats:p>","DOI":"10.1002\/dneu.20705","type":"journal-article","created":{"date-parts":[[2009,2,17]],"date-time":"2009-02-17T20:17:47Z","timestamp":1234901867000},"page":"299-313","source":"Crossref","is-referenced-by-count":13,"title":["Age\u2010dependent differential expression of genes involved in steroid signalling pathway in the brain of protandrous black porgy, <i>Acanthopagrus 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