{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:09:29Z","timestamp":1792570169505,"version":"4.5.0"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,11,2]],"date-time":"2009-11-02T00:00:00Z","timestamp":1257120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Localized changes in the composition of axonal cytoplasm (axoplasm) are critical for many biological processes, including axon guidance, responses to injury, neurite outgrowth, and axon\u2010glia interactions. Biochemical and molecular studies of these mechanisms have been heavily focused on <jats:italic>in vitro<\/jats:italic> systems because of the difficulty of obtaining subcellular extracts from mammalian tissues <jats:italic>in vivo<\/jats:italic>. As <jats:italic>in vitro<\/jats:italic> systems might not replicate the <jats:italic>in vivo<\/jats:italic> situation, reliable methods of axoplasm extraction from whole nerve would be helpful for mechanistic studies on axons. Here we develop and evaluate a new procedure for preparation of axoplasm from rat peripheral nerve, based on incubation of separated short segements of nerve fascicles in hypotonic medium to separate myelin and lyse nonaxonal structures, followed by extraction of the remaining axon\u2010enriched material. We show that this new procedure reduces serum and glial cell contamination and facilitates proteomic analyses of axonal contents. \u00a9 2009 Wiley Periodicals, Inc. Develop Neurobiol, 2010<\/jats:p>","DOI":"10.1002\/dneu.20755","type":"journal-article","created":{"date-parts":[[2009,11,2]],"date-time":"2009-11-02T19:23:12Z","timestamp":1257189792000},"page":"126-133","source":"Crossref","is-referenced-by-count":37,"title":["Axoplasm isolation from peripheral nerve"],"prefix":"10.1002","volume":"70","author":[{"given":"Ida","family":"Rishal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izhak","family":"Michaelevski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meir","family":"Rozenbaum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Shinder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin F.","family":"Medzihradszky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alma L.","family":"Burlingame","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Fainzilber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,11,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800389"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970050203"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2429-08.2008"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.175.4028.1370"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.04089.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00770-0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471143030.cb0325s33"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1974.tb10763.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2004.05.009"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.01.023"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M400004-MCP200"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/BA20080028"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-09-04064.1993"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr070293w"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2009.02.007"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0611562104"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2009.06.002"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.20511"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4235-04.2005"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200703209"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200902146"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1773"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.05.029"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-23-09291.2001"}],"container-title":["Developmental Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdneu.20755","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dneu.20755","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T20:29:23Z","timestamp":1700598563000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dneu.20755"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11,2]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["10.1002\/dneu.20755"],"URL":"https:\/\/doi.org\/10.1002\/dneu.20755","archive":["Portico"],"relation":{},"ISSN":["1932-8451","1932-846X"],"issn-type":[{"value":"1932-8451","type":"print"},{"value":"1932-846X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,11,2]]}}}