{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:40:11Z","timestamp":1786977611181,"version":"build-2736575974"},"reference-count":62,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,4]],"date-time":"2013-11-04T00:00:00Z","timestamp":1383523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cerebellar Purkinje cells have the most elaborate dendritic trees among neurons in the brain. We examined the roles of ryanodine receptor (RyR), an intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release channel, in the dendrite formation of Purkinje cells using cerebellar cell cultures. In the cerebellum, Purkinje cells express RyR1 and RyR2, whereas granule cells express RyR2. When ryanodine (10 \u00b5\n                    <jats:italic>M<\/jats:italic>\n                    ), a blocker of RyR, was added to the culture medium, the elongation and branching of Purkinje cell dendrites were markedly inhibited. When we transferred small interfering RNA (siRNA) against\n                    <jats:italic>RyR1<\/jats:italic>\n                    into Purkinje cells using single\u2010cell electroporation, dendritic branching but not elongation of the electroporated Purkinje cells was inhibited. On the other hand, transfection of\n                    <jats:italic>RyR2<\/jats:italic>\n                    siRNA into granule cells also inhibited dendritic branching of Purkinje cells. Furthermore, ryanodine reduced the levels of brain\u2010derived neurotrophic factor (BDNF) in the culture medium. The ryanodine\u2010induced inhibition of dendritic differentiation was partially rescued when BDNF was exogenously added to the culture medium in addition to ryanodine. Overall, these results suggest that RyRs expressed by both Purkinje and granule cells play important roles in promoting the dendritic differentiation of Purkinje cells and that RyR2 expressed by granule cells is involved in the secretion of BDNF from granule cells. \u00a9 2013 Wiley Periodicals, Inc. Develop Neurobiol 74: 467\u2013480, 2014\n                  <\/jats:p>","DOI":"10.1002\/dneu.22139","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T22:35:26Z","timestamp":1381530926000},"page":"467-480","source":"Crossref","is-referenced-by-count":24,"title":["Dendritic differentiation of cerebellar Purkinje cells is promoted by ryanodine receptors expressed by Purkinje and granule cells"],"prefix":"10.1002","volume":"74","author":[{"given":"Ryo","family":"Ohashi","sequence":"first","affiliation":[{"name":"Department of Cellular Biophysics Graduate School of Pharmaceutical Sciences, Nagoya City University Nagoya 467\u20108603 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin\u2010ichi","family":"Sakata","sequence":"additional","affiliation":[{"name":"Department of Cellular Biophysics Graduate School of Pharmaceutical Sciences, Nagoya City University Nagoya 467\u20108603 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