{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T07:44:18Z","timestamp":1696664658232},"reference-count":92,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,6,8]],"date-time":"2015-06-08T00:00:00Z","timestamp":1433721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The University of Bari, Italy, from the Italian Ministry of the Education, University and Research","award":["Miur, PRIN 2009; 2009TBCZJB_002"],"award-info":[{"award-number":["Miur, PRIN 2009; 2009TBCZJB_002"]}]},{"name":"The Regione Puglia","award":["PON project: D.A.R.E. Proalifun; PON02_00186_2937475"],"award-info":[{"award-number":["PON project: D.A.R.E. Proalifun; PON02_00186_2937475"]}]},{"name":"The European Union PON Infrastructures"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:bold>The Contactin\u20101 axonal glycoprotein (formerly F3\/Contactin) plays a relevant role in cerebellar ontogenesis, as shown in Contactin\u20101 KO\u2010mice and in transgenic mice misexpressing the corresponding cDNA from a heterologous promoter. Likewise, null mutant mice for the Collier\/Olf1\/Early B\u2010cell family transcription factor EBF2, in which Purkinje neuron development is primarily affected, exhibit abnormalities in cerebellar corticogenesis. Here, to evaluate the contribution to the <jats:italic>Ebf2<\/jats:italic> null phenotype of changes in the profile of Contactin\u20101, we study its expression in <jats:italic>Ebf2<\/jats:italic> null mice. In addition, we explore the activation profile of the <jats:italic>Cntn1<\/jats:italic> gene promoter upon transferring the <jats:italic>Ebf2<\/jats:italic> mutation to transgenic mice expressing an enhanced green fluorescent protein reporter under control of <jats:italic>Cntn1<\/jats:italic> gene regulatory sequences. In <jats:italic>Ebf2<\/jats:italic> null mice, Contactin\u20101 protein expression and <jats:italic>Cntn1<\/jats:italic> gene promoter activity are both downregulated during embryonic and early postnatal cerebellar development, both in the rostral and caudal folia, while in the latter an upregulation is observed at postnatal day 8. <jats:italic>In vitro<\/jats:italic>, vectors driving EBF1,2,3 transcription factors from a cytomegalovirus (CMV)<\/jats:bold> <jats:bold>promoter transactivate a <jats:italic>Cntn1\u2010<\/jats:italic>Choline acetyltransferse (CAT) promoter\u2010reporter construct in cotransfection assays and, accordingly, by chromatin immunoprecipitation, we show that the <jats:italic>Cntn1<\/jats:italic> gene 5\u2032 flanking region is bound by the EBF2 transcription factor, consistent with the evidence that this region bears the cognate deoxyribonucleic acid (DNA) consensus sequences. These data indicate that Contactin\u20101 expression is dependent upon EBF factors, suggesting that the <jats:italic>Cntn1<\/jats:italic> gene belongs to the expanding regulatory cascade driven by these transcriptional regulators so that changes in its activation may contribute to the phenotype of <jats:italic>Ebf2<\/jats:italic> null mutant mice<\/jats:bold>. \u00a9 2015 Wiley Periodicals, Inc. Develop Neurobiol 75: 1420\u20131440, 2015<\/jats:p>","DOI":"10.1002\/dneu.22293","type":"journal-article","created":{"date-parts":[[2015,3,29]],"date-time":"2015-03-29T03:26:18Z","timestamp":1427599578000},"page":"1420-1440","source":"Crossref","is-referenced-by-count":4,"title":["The gene encoding the mouse contactin\u20101 axonal glycoprotein is regulated by the collier\/Olf1\/EBF family early <scp>B<\/scp>\u2010Cell factor 2 transcription factor"],"prefix":"10.1002","volume":"75","author":[{"given":"Antonella","family":"Bizzoca","sequence":"first","affiliation":[{"name":"Department of Basic Medical Sciences, Neurosciences and Sensory Organs Medical School, University of Bari Policlinico Bari I\u201070124 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina","family":"Picocci","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Neurosciences and Sensory Organs Medical School, University of Bari Policlinico Bari I\u201070124 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrizia","family":"Corsi","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Neurosciences and Sensory Organs Medical School, University of Bari Policlinico Bari I\u201070124 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania","family":"Arbia","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Neurosciences and Sensory Organs Medical School, University of Bari Policlinico Bari I\u201070124 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Croci","sequence":"additional","affiliation":[{"name":"Division of Neuroscience San Raffaele Scientific Institute  Milano I\u201020132 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Giacomo","family":"Consalez","sequence":"additional","affiliation":[{"name":"Division of Neuroscience San Raffaele Scientific Institute  Milano I\u201020132 Italy"},{"name":"Universit\u00e0 Vita\u2010Salute San Raffaele  Milano I\u201020132 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianfranco","family":"Gennarini","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Neurosciences and Sensory Organs Medical School, University of Bari Policlinico Bari I\u201070124 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902310103"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902310104"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12440"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2013.05.001"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81126-5"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.4161\/cam.3.1.7462"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.02.011"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00183"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00296-3"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1313769110"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(97)00100-9"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2008.01.090"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00215"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2010.78"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02437"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.14.3089"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2006.06.007"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(01)00243-1"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70084-3"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(01)00486-5"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.16.3585"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2899-04.2005"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5203-05.2006"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-011-0398-1"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00840"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199711)210:3<191::AID-AJA1>3.0.CO;2-B"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.2.775"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90062-5"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(98)01313-7"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1749-8104-6-19"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.2012.01348.x"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.102988"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2010.08.008"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2004.05.012"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/14734220600589202"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00810-9"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1159\/000090750"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2008.01.008"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290016"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10059-000-0001-0"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3650"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00182"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.23.9055-9067.2000"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt979"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-5748(00)00071-X"},{"key":"e_1_2_5_48_1","first-page":"110","article-title":"Distinct modes of neuronal migration in different domains of developing cerebellar cortex","volume":"37","author":"Komuro H","year":"1998","journal-title":"J Neurosci"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.5.3002"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108774108"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.03.007"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.24.8389-8397.2002"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00828-0"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0106"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2008.0346"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.28.17632"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2013.00018"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(02)00336-2"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2012.05.003"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.142869"},{"key":"e_1_2_5_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2011.07606.x"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-8090-7_8"},{"key":"e_1_2_5_63_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3493-06.2006"},{"key":"e_1_2_5_64_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-10-03563.1998"},{"key":"e_1_2_5_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2495"},{"key":"e_1_2_5_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.06.036"},{"key":"e_1_2_5_67_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054105"},{"key":"e_1_2_5_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2015.01.004"},{"key":"e_1_2_5_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.22186"},{"key":"e_1_2_5_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.04.001"},{"key":"e_1_2_5_71_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.26.041002.131014"},{"key":"e_1_2_5_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.200900063"},{"key":"e_1_2_5_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23203"},{"key":"e_1_2_5_74_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.29.051605.113034"},{"key":"e_1_2_5_75_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.21.1.127"},{"key":"e_1_2_5_76_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2005.tb00045.x"},{"key":"e_1_2_5_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2004.03.004"},{"key":"e_1_2_5_78_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240480110"},{"key":"e_1_2_5_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3264"},{"key":"e_1_2_5_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2012.01.010"},{"key":"e_1_2_5_81_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01143-12"},{"key":"e_1_2_5_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2009.12.006"},{"key":"e_1_2_5_83_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12311-011-0350-6"},{"key":"e_1_2_5_84_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19991025)413:3<357::AID-CNE1>3.0.CO;2-S"},{"key":"e_1_2_5_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80195-X"},{"key":"e_1_2_5_86_1","doi-asserted-by":"publisher","DOI":"10.1038\/35081558"},{"key":"e_1_2_5_87_1","first-page":"3","article-title":"Early mesencephalon\/metencephalon patterning and development of the cerebellum","volume":"5","author":"Wassef M","year":"1997","journal-title":"Perspect Dev Neurobiol"},{"key":"e_1_2_5_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80682-0"},{"key":"e_1_2_5_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(00)00177-X"},{"key":"e_1_2_5_90_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.051912"},{"key":"e_1_2_5_91_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2722-13.2014"},{"key":"e_1_2_5_92_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105245200"},{"key":"e_1_2_5_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90399-9"}],"container-title":["Developmental Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdneu.22293","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dneu.22293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T01:32:20Z","timestamp":1696642340000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dneu.22293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,8]]},"references-count":92,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1002\/dneu.22293"],"URL":"https:\/\/doi.org\/10.1002\/dneu.22293","archive":["Portico"],"relation":{},"ISSN":["1932-8451","1932-846X"],"issn-type":[{"value":"1932-8451","type":"print"},{"value":"1932-846X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,8]]}}}