{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:13:26Z","timestamp":1782278006826,"version":"3.54.5"},"reference-count":76,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,8,26]],"date-time":"2018-08-26T00:00:00Z","timestamp":1535241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["CoBRE P30 GM103503"],"award-info":[{"award-number":["CoBRE P30 GM103503"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01 DC007695 (GS)"],"award-info":[{"award-number":["R01 DC007695 (GS)"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neural circuit formation involves maturation of neuronal, glial and vascular cells, as well as cell proliferation and cell death. A fundamental understanding of cellular mechanisms is enhanced by quantification of cell types during key events in synapse formation and pruning and possessing qualified genetic tools for cell type\u2010specific manipulation. Acquiring this information in turn requires validated cell markers and genetic tools. We quantified changing proportions of neurons, astrocytes, oligodendrocytes, and microglia in the medial nucleus of the trapezoid body (MNTB) during neural circuit development. Cell type\u2010specific markers, light microscopy and 3D virtual reality software, the latter developed in our laboratory, were used to count cells within distinct cell populations at postnatal days (P)3 and P6, bracketing the period of nerve terminal growth and pruning in this system. These data revealed a change from roughly equal numbers of neurons and glia at P3 to a 1.5:1 ratio of glia to neurons at P6. PCNA and PH3 labeling revealed that proliferation of oligodendrocytes contributed to the increase in glial cell number during this timeframe. We next evaluated Cre driver lines for selectivity in labeling cell populations. <jats:italic>En1\u2010Cre<\/jats:italic> was specific for MNTB neurons. <jats:italic>PDGFR\u03b1\u2010Cre<\/jats:italic> and <jats:italic>Aldh1L1\u2010Cre<\/jats:italic>, thought to be mostly specific for oligodendrocyte lineage cells and astrocytes, respectively, both labeled significant numbers of neurons, oligodendrocytes, and astrocytes and are non\u2010specific genetic tools in this neural system.<\/jats:p>","DOI":"10.1002\/dneu.22633","type":"journal-article","created":{"date-parts":[[2018,8,23]],"date-time":"2018-08-23T08:10:31Z","timestamp":1535011831000},"page":"1097-1116","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Glial Cell Expansion Coincides with Neural Circuit Formation in the Developing Auditory Brainstem"],"prefix":"10.1002","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9862-0977","authenticated-orcid":false,"given":"Ashley N.","family":"Brandebura","sequence":"first","affiliation":[{"name":"Rockefeller Neuroscience Institute West Virginia University Morgantown West Virginia"},{"name":"Sensory Neuroscience Research Center West Virginia University Morgantown West Virginia"},{"name":"Graduate program in Biochemistry and Molecular Biology West Virginia University Morgantown West Virginia"},{"name":"Department of Biochemistry West Virginia University Morgantown West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Morehead","sequence":"additional","affiliation":[{"name":"Rockefeller Neuroscience Institute West Virginia University Morgantown West Virginia"},{"name":"Sensory Neuroscience Research Center West Virginia University Morgantown West Virginia"},{"name":"Lane Department of Computer Science and Electrical Engineering West Virginia University Morgantown West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel T.","family":"Heller","sequence":"additional","affiliation":[{"name":"Rockefeller Neuroscience Institute West Virginia University Morgantown West Virginia"},{"name":"Sensory Neuroscience Research Center West Virginia University Morgantown West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Holcomb","sequence":"additional","affiliation":[{"name":"Rockefeller Neuroscience Institute West Virginia University Morgantown West Virginia"},{"name":"Sensory Neuroscience Research Center West Virginia University Morgantown West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas R.","family":"Kolson","sequence":"additional","affiliation":[{"name":"Rockefeller Neuroscience Institute West Virginia University Morgantown West Virginia"},{"name":"Sensory Neuroscience Research Center West Virginia University Morgantown West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garrett","family":"Jones","sequence":"additional","affiliation":[{"name":"Rockefeller Neuroscience Institute West Virginia University Morgantown West Virginia"},{"name":"Sensory Neuroscience Research Center West Virginia University Morgantown West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter H.","family":"Mathers","sequence":"additional","affiliation":[{"name":"Rockefeller Neuroscience Institute West Virginia University Morgantown West Virginia"},{"name":"Sensory Neuroscience Research Center West Virginia University Morgantown West Virginia"},{"name":"Department of Biochemistry West Virginia University Morgantown West Virginia"},{"name":"Department of Otolaryngology HNS West Virginia University Morgantown West Virginia"},{"name":"Department of Ophthalmology West Virginia University Morgantown West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George A.","family":"Spirou","sequence":"additional","affiliation":[{"name":"Rockefeller Neuroscience Institute West Virginia University Morgantown West Virginia"},{"name":"Sensory Neuroscience Research Center West Virginia University Morgantown West Virginia"},{"name":"Department of Otolaryngology HNS West Virginia University Morgantown West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2015.10.008"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.22388"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21179"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21974"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-07-02819.1992"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804650106"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21449"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00574-8"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.20702"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4178-07.2008"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.22424"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12776"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.2004.00170.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013741"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2014.08.016"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019849"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.490508"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.09.053"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.010454"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22539"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00249"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20776"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)90644-6"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22683"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5525-05.2006"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.198564"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1087-13.2013"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22648"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(98)00040-0"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2604-13.2013"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903280202"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.09.009"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22409"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1620"},{"key":"e_1_2_6_36_1","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1101\/gad.14.11.1377","article-title":"Two lineage boundaries coordinate vertebrate apical ectodermal ridge formation","volume":"14","author":"Kimmel R.A.","year":"2000","journal-title":"Genes & Development"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.22306"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23204"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027383"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.051508.135600"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1104977108"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(02)00374-X"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903140406"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2467"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.24.5253"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2232-09.2009"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.22069"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(68)90235-7"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19960201)43:3<299::AID-JNR5>3.0.CO;2-E"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/35055553"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1172"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(78)90105-1"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.1200"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1202529"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/embr.201438447"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2007.04.013"},{"key":"e_1_2_6_57_1","unstructured":"PidhorskyiS. MoreheadM. JonesQ. SpirouG.andDorettoG. (Submitted)syGlass: interactive exploration of multidimensional images using virtual reality head\u2010mounted displays."},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2007.02.010"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2008.01.012"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200910354"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3516-10.2011"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2220"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20918"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21730"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.20856"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23473"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907143106"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neunet.2008.05.004"},{"key":"e_1_2_6_69_1","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1242\/dev.126.19.4201","article-title":"Engrailed\u20101 and netrin\u20101 regulate axon pathfinding by association interneurons that project to motor neurons","volume":"126","author":"Saueressig H.","year":"1999","journal-title":"Development"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02463354"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.03.026"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903440107"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)60679-2"},{"key":"e_1_2_6_74_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.077214"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.09.043"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad3839"},{"key":"e_1_2_6_77_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.004895"}],"container-title":["Developmental Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdneu.22633","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dneu.22633","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T14:51:06Z","timestamp":1694875866000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dneu.22633"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,26]]},"references-count":76,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1002\/dneu.22633"],"URL":"https:\/\/doi.org\/10.1002\/dneu.22633","archive":["Portico"],"relation":{},"ISSN":["1932-8451","1932-846X"],"issn-type":[{"value":"1932-8451","type":"print"},{"value":"1932-846X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,26]]},"assertion":[{"value":"2017-12-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}