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The role of PTHrP in spinal cord development has not been characterized. <jats:italic>Pthrp<\/jats:italic> knock in (<jats:italic>Pthrp<\/jats:italic> KI) mice were genetically modified to produce PTHrP in which there is a deficiency of the nuclear localization sequence (NLS) and C\u2010terminus. Using this genetically modified mouse model, we have characterized its effect on spinal cord development early postnatally. The spinal cords from <jats:italic>Pthrp<\/jats:italic> KI mice displayed a significant reduction in its length, weight, and cross\u2010sectional area compared to wild\u2010type controls. Histologically, there was a decreased development of neurons and glial cells that caused decreased cell proliferation and increased apoptosis. The neural stem cells (NSCs) cultures also revealed decreased cell proliferation and differentiation and increased apoptosis. The proposed mechanism of delayed spinal cord development in <jats:italic>Pthrp<\/jats:italic> KI mice may be due to alteration in associated pathways in regulation of cell\u2010division cycles and apoptosis. There was significant downregulation of Bmi\u20101 and upregulation of cyclin\u2010dependent kinase inhibitors p27, p21, and p16 in <jats:italic>Pthrp<\/jats:italic> KI animals. We conclude that NLS and C\u2010terminus peptide segments of PTHrP play an important role in inhibiting cell apoptosis and stimulation of cellular proliferation necessary for normal spinal cord development.<\/jats:p>","DOI":"10.1002\/dneu.22798","type":"journal-article","created":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T20:05:03Z","timestamp":1607112303000},"page":"47-62","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Role of PTHrP nuclear localization and carboxyl terminus sequences in postnatal spinal cord development"],"prefix":"10.1002","volume":"81","author":[{"given":"Yahong","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Human Anatomy Nanjing Medical University Nanjing P.R. 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