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Does it work as a potential deaf gene to regulate hair cell development? We used whole\u2010mount in situ hybridization (WISH) assay and morpholino\u2010mediated gene knockdown method to investigate the role of <jats:italic>irf7<\/jats:italic> in the development of otic vesicle hair cells during zebrafish embryogenesis. We performed RNA sequencing to gain a detailed insight into the molecules\/genes which are altered upon downregulation of <jats:italic>irf7<\/jats:italic>. Compared to the wild\u2010type siblings, knockdown of <jats:italic>irf7<\/jats:italic> resulted in severe developmental retardation in zebrafish embryos as well as loss of neuromasts and damage to hair cells at an early stage (within 3 days post fertilization). Coinjection of zebrafish <jats:italic>irf7<\/jats:italic> mRNA could partially rescued the defects of the morphants. <jats:italic>atp1b2b<\/jats:italic> mRNA injection can also partially rescue the phenotype induced by <jats:italic>irf7<\/jats:italic> gene deficiency. Loss of hair cells in <jats:italic>irf7<\/jats:italic>\u2010morphants does not result from cell apoptosis. Gene expression profiles show that, compared to wild\u2010type, knockdown of <jats:italic>irf7<\/jats:italic> can lead to 2053 and 2678 genes being upregulated and downregulated, respectively. Among them, 18 genes were annotated to hair cell (HC) development or posterior lateral line (PLL) development. All results suggest that <jats:italic>irf7<\/jats:italic> plays an essential role in hair cell development in zebrafish, indicating that <jats:italic>irf7<\/jats:italic> may be a member of deafness gene family.<\/jats:p>","DOI":"10.1002\/dneu.22860","type":"journal-article","created":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T07:47:18Z","timestamp":1636962438000},"page":"88-97","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Interferon regulatory factor\u20107 is required for hair cell development during zebrafish embryogenesis"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7944-7985","authenticated-orcid":false,"given":"Song\u2010Qun","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology The First Affiliated Hospital Nanjing Medical University  Nanjing China"},{"name":"Department of Otorhinolaryngology Affiliated Hospital of 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