{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T18:13:46Z","timestamp":1778782426946,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T00:00:00Z","timestamp":1748217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T00:00:00Z","timestamp":1748217600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In this study we propose a cell\u2010culture protocol to better comprehend the involvement of immune\/blood cells (hemocytes) in the adult neurogenesis of crustaceans. We examined whether the hemocytes of the crab\n                    <jats:italic>Ucides cordatus<\/jats:italic>\n                    may develop into neural cells in response to an in vitro stimulus. The experiments involved two steps. First, we selected an appropriate substrate for use in the culture medium. Hemocytes proliferated and differentiated most on poly\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010lysine. Second, we added pituitary extract to the poly\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010lysine\u2010coated culture in order to determine the cell types into which hemocytes differentiated. This supplement was chosen due to its mitogenic and cell\u2010differentiation properties. Using cell type\u2010specific antibodies (anti\u2010GFAP, anti\u2010vimentin, anti\u2010beta III Tubulin, and anti\u2010NeuN), we were able to identify putative neural progenitors. This showed that upon stimulation, hemocytes have mitotic activity and can display neural precursor proteins. In contrast to the protocols commonly used for vertebrate cell cultures, the protocol used here proved, for the first time, to be capable of stimulating crustacean blood cells to grow. This method also offers a basis for cultivating crustacean blood cells for various purposes, such as the study of adult neurogenesis.\n                  <\/jats:p>","DOI":"10.1002\/dneu.22972","type":"journal-article","created":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T04:35:16Z","timestamp":1748234116000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mangrove\u2010Crab Blood Cells Proliferate In Vitro and Display Neuronal Proteins Following Pituitary\u2010Extract Stimulus"],"prefix":"10.1002","volume":"85","author":[{"given":"In\u00eas J\u00falia Ribas","family":"Wajsenzon","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Morfol\u00f3gicas, Instituto de Ci\u00eancias Biom\u00e9dicas Universidade Federal do Rio de Janeiro  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isadora Santos de","family":"Abreu","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas (Fisiologia), Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Augusto Borges de Andrade","family":"Gomes","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas (Biof\u00edsica), Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wagner Ant\u00f4nio Barbosa da","family":"Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas (Fisiologia), Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriano","family":"Biancalana","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ensino de Ci\u00eancias, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal do Par\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Giestal\u2010de\u2010Araujo","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Neuroci\u00eancias, Instituto de Biologia Universidade Federal Fluminense  Niter\u00f3i Rio de Janeiro Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Neuroimunomodula\u00e7\u00e3o (INCT\u2010NIM)  Manguinhos Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3053-3866","authenticated-orcid":false,"given":"Silvana","family":"Allodi","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Morfol\u00f3gicas, Instituto de Ci\u00eancias Biom\u00e9dicas Universidade Federal do Rio de Janeiro  Rio de Janeiro Rio de Janeiro Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas (Fisiologia), Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Rio de Janeiro Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas (Biof\u00edsica), Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2021.110570"},{"key":"e_1_2_11_3_1","first-page":"1","article-title":"Hypersecretion of Mucus Induced in Isolated Non\u2010Innervated Cells","volume":"12","author":"Bang B. 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