{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:09:46Z","timestamp":1787162986875,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T00:00:00Z","timestamp":1750291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T00:00:00Z","timestamp":1750291200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Perineuronal nets (PNNs) are specialized extracellular matrix structures that surround certain neurons and play a critical role in protecting neurons from oxidative stress and maintaining synaptic stability in the central nervous system. They have roles in memory formation, and their loss has been linked to various mental alterations, such as anxiety, depression, and schizophrenia. While immune activation is known to degrade PNNs, it remains unclear whether inflammasomes are involved in PNN formation dynamics during neuronal development, where cases of sepsis are particularly high. In this study, we investigated how activation of the NLRP3 inflammasome in neonatal mouse brains influences PNNs. To explore this, neonatal wild\u2010type and\n                    <jats:italic>Nlrp3<\/jats:italic>\n                    knockout mice were injected with lipopolysaccharide (LPS) or phosphate\u2010buffered saline (PBS) on postnatal day (PND) 9, and PNNs were visualized at early adulthood (PND60). In addition, NLRP3 inflammasome activation was confirmed on PND10, and behavioral tests were performed on PND60. LPS treatment in wild\u2010type mice reduced PNN\u2010positive neurons in the hippocampus and cortex compared to the PBS group, whereas\n                    <jats:italic>Nlrp3<\/jats:italic>\n                    knockout mice showed no differences between treatment groups. Moreover, behavioral tests revealed that neonatal LPS injection resulted in anxiety\u2010 and depressive\u2010like behavior and that NLRP3 deficiency restrained this effect. These results highlight the key role of NLRP3 inflammasome activation in inflammation\u2010driven PNN reduction during neuronal development. NLRP3 inhibitors could thus serve as potential therapeutic agents to protect the neuronal extracellular matrix from inflammatory damage in early life.\n                  <\/jats:p>","DOI":"10.1002\/dneu.22986","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T02:14:31Z","timestamp":1750385671000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Neonatal NLRP3 Inflammasome Activation Leads to Perineuronal Net Deficits in Early Adulthood"],"prefix":"10.1002","volume":"85","author":[{"given":"Emre","family":"Tarakcioglu","sequence":"first","affiliation":[{"name":"Izmir Biomedicine and Genome Center Izmir Turkey"},{"name":"Izmir International Biomedicine and Genome Institute Dokuz Eylul University Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bilgesu","family":"Genc","sequence":"additional","affiliation":[{"name":"Izmir Biomedicine and Genome Center 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