{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T09:29:25Z","timestamp":1786699765222,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The capacity for enhanced experience, modeled as environmental enrichment (EE) in laboratory animals, to drive positive changes in brain circuitry presents a promising avenue in the development of therapies for neurodevelopmental conditions. Less understood are the underlying mechanisms, or potential interactions of EE with other therapeutic approaches. We have previously shown that early exposure to EE can drive the partial repair of miswired uncrossed retinal projections, and the concomitant rescue of a visual behavior, in the Ten\u2010m3 knockout (KO) mouse. This was associated with a highly spatiotemporally localized upregulation of microglial reactivity in the region where the correction was occurring which peaked around postnatal day (P)25. Aiming to confirm a causal role for microglial\u2010mediated engulfment in this process, we assessed the effect of daily injections of minocycline or vehicle saline from P18 to P24 (inclusive) on measures of microglial reactivity and anatomical corrective pruning in P25 Ten\u2010m3 KO mice. While an effect of EE was confirmed at this timepoint, intriguingly, we found that both the vehicle\u2010 and minocycline\u2010treated mice had a similar lack of microglial reactivity and showed a marked absence of corrective pruning of their miswired retinal projections. This suggests that the injection procedure itself disrupted the experience\u2010induced microglial\u2010mediated circuit repair. These results underscore the highly sensitive nature of EE\u2010driven corrective pruning actions of microglia and the critical importance of considering and controlling for all aspects of experience.<\/jats:p>","DOI":"10.1002\/dneu.70003","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T08:36:49Z","timestamp":1768293409000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Daily Injection Paradigm Disrupts the Environmental\u2010Enrichment\u2010Induced Pruning of Miswired Axonal Projections in Developing Mice"],"prefix":"10.1002","volume":"86","author":[{"given":"Lara","family":"Rogerson\u2010Wood","sequence":"first","affiliation":[{"name":"Neuroscience, School of Medical Sciences, Faculty of Medicine and Health University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atomu","family":"Sawatari","sequence":"additional","affiliation":[{"name":"Neuroscience, School of Medical Sciences, Faculty of Medicine and Health University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire S.","family":"Goldsbury","sequence":"additional","affiliation":[{"name":"Neuroscience, School of Medical Sciences, Faculty of Medicine and Health University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A.","family":"Leamey","sequence":"additional","affiliation":[{"name":"Neuroscience, School of Medical Sciences, Faculty of Medicine and Health University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3541\u201012.2013"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyg.2019.00466"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awy111"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","unstructured":"Biot E. 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