{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T07:04:29Z","timestamp":1785135869992,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82360257"],"award-info":[{"award-number":["82360257"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Spinal cord injury (SCI) is a serious neurological disorder for which there is currently no effective treatment. Telomerase reverse transcriptase (\n TERT<\/jats:italic>\n ), as the catalytic subunit of telomerase, is closely related to the proliferation and differentiation of astrocytes. This study aims to explore the mechanism of the\n TERT<\/jats:italic>\n gene in promoting SCI repair and regeneration by inducing reactive astrocytes (RAs) and its potential application as a therapeutic target. First, the RAs model of\n TERT<\/jats:italic>\n gene overexpression was constructed using a lentiviral vector, and its efficiency was verified using qRT\u2010PCR and Western blotting. The effect of\n TERT<\/jats:italic>\n expression on the proliferation and apoptosis of RAs was detected using CCK8 and flow cytometry assays. Subsequently, immunofluorescence and Western blotting were used to detect changes in the expression of proteins, such as\n NFL<\/jats:italic>\n ,\n Nestin<\/jats:italic>\n ,\n NSE<\/jats:italic>\n , and\n GFAP<\/jats:italic>\n , as well as their protein interactions with downstream pathways, such as\n GSK\u20103\u03b2<\/jats:italic>\n ,\n Klf4<\/jats:italic>\n , and\n \u03b2\u2010catenin<\/jats:italic>\n , to further explore the specific mechanism of the\n TERT<\/jats:italic>\n gene in SCI regeneration and repair. CCK8 assay showed that the survival rate of RAs increased with the upregulation of\n TERT<\/jats:italic>\n expression. Flow cytometry assay showed that\n TERT<\/jats:italic>\n expression was negatively correlated with the apoptosis rate of RAs. Immunofluorescence showed that the expression of proteins, such as\n NFL<\/jats:italic>\n ,\n GFAP<\/jats:italic>\n , and\n Nestin<\/jats:italic>\n , increased with the upregulation of\n TERT<\/jats:italic>\n expression, suggesting that\n TERT<\/jats:italic>\n upregulation is involved in the proliferation and neural differentiation of RAs. Furthermore, western blotting showed that after\n TERT<\/jats:italic>\n expression was upregulated,\n GSK\u20103\u03b2<\/jats:italic>\n expression was significantly downregulated, whereas\n Klf4<\/jats:italic>\n ,\n \u03b2\u2010catenin<\/jats:italic>\n ,\n Nestin<\/jats:italic>\n , and\n NSE<\/jats:italic>\n were significantly upregulated compared with the control group. Upregulation of\n TERT<\/jats:italic>\n expression enhances the proliferation of RAs and inhibits their apoptosis. The expression level of\n TERT<\/jats:italic>\n is positively correlated with the proliferation and neural differentiation of RAs. In addition, these findings suggest that\n TERT<\/jats:italic>\n can promote the proliferation and differentiation of RAs by regulating the\n Wnt<\/jats:italic>\n \/\n \u03b2\u2010catenin<\/jats:italic>\n pathway, demonstrating the potential of\n TERT<\/jats:italic>\n as a therapeutic target for SCI.\n <\/jats:p>","DOI":"10.1002\/dneu.70040","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T07:16:31Z","timestamp":1778829391000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["TERT Promotes Reactive Astrocytes Proliferation and Differentiation Through the Wnt\/\u03b2\u2010Catenin Pathway and Improves Spinal Cord Injury"],"prefix":"10.1002","volume":"86","author":[{"given":"Shutao","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Spine Surgery First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizhe","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukun","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weibin","family":"Sheng","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,15]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.642719"},{"issue":"20","key":"e_1_2_12_3_1","first-page":"11471","article-title":"The Wnt\/\u03b2\u2010Catenin Signaling Pathway Inhibits Osteoporosis by Regulating the Expression of TERT: An In Vivo and In Vitro Study","volume":"15","author":"Cai Y.","year":"2023","journal-title":"Aging (Albany NY)"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-023-07561-3"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-025-01240-3"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00783-4"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202401227"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-024-00342-8"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2024.102648"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.70244"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.advnut.2025.100448"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.06.006"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2024.06.003"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-024-06454-7"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-024-03514-9"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2024.102598"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-024-00823-y"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2024.189243"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0565-5"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2023.0438"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-024-01563-4"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.10.118"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2025.106854"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-013-1097-x"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2025.102773"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-024-04134-3"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-025-02363-1"},{"issue":"11","key":"e_1_2_12_28_1","first-page":"810","article-title":"Telomerase Expression in the Glial Scar of Rats With Spinal Cord Injury","volume":"7","author":"Yang M.","year":"2012","journal-title":"Neural Regeneration Research"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1165632"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X25500272"}],"container-title":["Developmental Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dneu.70040","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dneu.70040","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dneu.70040","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T06:51:44Z","timestamp":1785135104000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dneu.70040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,15]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/dneu.70040"],"URL":"https:\/\/doi.org\/10.1002\/dneu.70040","archive":["Portico"],"relation":{},"ISSN":["1932-8451","1932-846X"],"issn-type":[{"value":"1932-8451","type":"print"},{"value":"1932-846X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,15]]},"assertion":[{"value":"2025-11-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70040"}}