{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T07:04:31Z","timestamp":1785135871269,"version":"3.55.0"},"reference-count":56,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Autism spectrum disorder (ASD) arises from interactions between genetic predisposition and prenatal environmental insults. Prostaglandin E\n                    <jats:sub>2<\/jats:sub>\n                    (PGE\n                    <jats:sub>2<\/jats:sub>\n                    ), derived from cyclooxygenase\u20102 (COX\u20102), regulates neuronal differentiation and glia\u2013neuron signaling; thus, its inhibition during gestation may impair neurodevelopment. To examine whether prenatal exposure to indomethacin, a non\u2010selective COX inhibitor, induces autism\u2010like alterations in rats with possible sex differences. Sixteen pregnant Wistar rats received indomethacin (1\u00a0mg\/kg, gavage) on Gestational Days 10\u201314. Adult offspring (P50\u2013P54) underwent behavioral tests (open\u2010field, rearing, rotarod, and three\u2010chamber sociability). Hippocampal and cerebellar tissues were analyzed for PGE\n                    <jats:sub>2<\/jats:sub>\n                    , LC3B (ELISA), MDA (TBARS), neuronal density, and GFAP expression (CA1, CA3 \u2013 Nissl, GFAP staining). Indomethacin reduced sociability, exploration, and motor performance, especially in males, and decreased PGE\n                    <jats:sub>2<\/jats:sub>\n                    and LC3B while increasing MDA (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001). Histologically, CA1 neuronal counts increased while GFAP immunoreactivity was markedly elevated in hippocampal (CA1 and CA3) and cerebellar regions (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05), reflecting astroglial activation and neuroinflammatory remodeling. Prenatal inhibition of prostaglandin synthesis may disturb PGE\n                    <jats:sub>2<\/jats:sub>\n                    signaling, leading to oxidative stress, altered autophagy\u2010related signaling, and glial activation, which together contribute to ASD\u2010relevant behavioral outcomes.\n                  <\/jats:p>","DOI":"10.1002\/dneu.70041","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T07:19:46Z","timestamp":1778829586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prenatal Indomethacin Exposure Is Associated With Autism\u2010Relevant Behavioral Alterations Linked to Oxidative Stress and Altered Autophagy\u2010Related Signaling"],"prefix":"10.1002","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2480-1374","authenticated-orcid":false,"given":"Bakiye","family":"Akba\u015f","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Medical Faculty Karadeniz Technical University  Trabzon Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6333-4919","authenticated-orcid":false,"given":"Ahmet","family":"Akba\u015f","sequence":"additional","affiliation":[{"name":"Department of General Surgery Faculty of Medicine Karadeniz Technical University  Trabzon Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3906-2538","authenticated-orcid":false,"given":"Nadir Adnan","family":"Hac\u0131m","sequence":"additional","affiliation":[{"name":"Department of General Surgery G\u00fcne\u015fli Erdem Hospital  \u0130stanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8420-9298","authenticated-orcid":false,"given":"G\u00fcl\u00e7in","family":"Ercan","sequence":"additional","affiliation":[{"name":"Department of General Surgery Sultan 2. Abdulhamid Han Educational and Research Hospital, Istanbul Provincial Directorate of Health  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatice","family":"Ayg\u00fcn","sequence":"additional","affiliation":[{"name":"Neuroscience Laboratory BAMER, Biruni University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00fcmin Alper","family":"Erdo\u011fan","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Medicine Izmir Katip Celebi University  Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u011fit","family":"Uyan\u0131kgil","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Faculty of Medicine Ege University  Izmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5427-8428","authenticated-orcid":false,"given":"Oytun","family":"Erba\u015f","sequence":"additional","affiliation":[{"name":"Faculty of Medicine BAMER, Biruni University  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1734-1140(11)70519-9"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1254"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1176\/appi.books.9780890425787"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-977X(01)00102-X"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2017.07.002"},{"issue":"1","key":"e_1_2_11_7_1","first-page":"14","article-title":"Indomethacin During Early Perinatal Age is 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