{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:06:26Z","timestamp":1791378386768,"version":"4.3.2"},"reference-count":67,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005771","name":"Dokuz Eyl\u00fcl \u00dcniversitesi","doi-asserted-by":"publisher","award":["ADEP TSA 2023\u20103026"],"award-info":[{"award-number":["ADEP TSA 2023\u20103026"]}],"id":[{"id":"10.13039\/501100005771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Neurobiology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Organoids are self\u2010organized, in vitro 3D structures derived from progenitor or pluripotent stem cells. During culture, these tissues secrete small extracellular vesicles (sEVs)\u2014functional bioactive molecules\u2014into their microenvironment. Here, we differentiated iPSC\u2010derived cerebral organoids (COs) under three culture conditions (static, orbital shaker, and a novel dynamic Organoid\u2010on\u2010a\u2010Chip platform) over 30 days of maturation to determine how culture dynamics shape organoid development and EV profiles. Transcriptomic analysis revealed strong, culture condition\u2010specific modulation of gene expression, while EV proteomics showed similar profiles across conditions at early maturation stages but marked divergence at later stages, reflecting differences in cellular maintenance, stress response, and tissue\u2010specific maturation pathways. Additionally, sEVs from all conditions reduced U87 glioblastoma cell viability and migration, with the strongest effect observed for sEVs derived from the orbital shaker condition. These findings demonstrate that organoid culture microenvironment substantially influences both developmental trajectory and the bioactivity of secreted EVs.<\/jats:p>","DOI":"10.1002\/dneu.70062","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:42:03Z","timestamp":1790660523000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular and Proteomic Profiling of Human Cerebral Organoid\u2011Derived Small Extracellular Vesicles"],"prefix":"10.1002","volume":"86","author":[{"given":"Elifsu","family":"Polatl\u0131","sequence":"first","affiliation":[{"name":"Izmir International Biomedicine and Genome Institute Dokuz Eyl\u00fcl University  \u0130zmir T\u00fcrkiye"},{"name":"Izmir Biomedicine and Genome Center  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burak","family":"Kahveci","sequence":"additional","affiliation":[{"name":"Izmir 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