{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:00:41Z","timestamp":1790694041720,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2012,7,31]],"date-time":"2012-07-31T00:00:00Z","timestamp":1343692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>This paper discusses the analytical challenges to develop a generic extraction procedure to analyze or screen multi\u2010class veterinary drugs in milk and honey using ultra\u2010high pressure liquid chromatography quadrupole time\u2010of\u2010flight mass spectrometry (UHPLC QqTOF MS). The veterinary drugs in this study included aminoglycosides, endectocides, fluoroquinolones, ionophores, \u03b2\u2010lactams or penicillins, macrolides, NSAIDs, phenicols, sulfonamides and tetracyclines. Veterinary drugs were extracted using a QuEChERS (quick, easy, cheap, effective, rugged, and safe) method, which entailed the use of acetonitrile containing 1% acetic acid, sodium acetate, ethylenediaminetetra acetic acid disodium (EDTA) and magnesium sulfate, and no clean\u2010up was performed. Chromatographic separation was achieved on a reversed\u2010phase Acquity UPLC BEH C<jats:sub>18<\/jats:sub>, 100\u2009\u00d7\u20092.1\u2009mm, 1.7\u2009\u00b5m column with 0.1% formic acid and 10\u2009mM ammonium formate in water, and acetonitrile as mobile phases. Due to poor chromatographic retention, aminoglycosides were first dropped from the list, and because of poor extractability, \u03b2\u2010lactams and tetracyclines were also excluded from the method. The method was able to quantify 31 or screen up to 54 drugs (unbound) in honey, and to quantify 34 or screen up to 59 drugs in milk. UHPLC QqTOF data were acquired in TOF MS full\u2010scan mode that allowed both quantification and confirmation of veterinary drugs and identification of their degradation products in samples. The method could achieve detection limits as low as 1\u2009\u00b5g\/kg with analytical range from 1 to 100\u2009\u00b5g\/kg. The developed method was intended to be used for screening of as many analytes as possible in one single analysis, or unequivocal confirmation of positive findings and degradation product identification based on accurate mass measurement and isotopic patterns. \u00a9 Her Majesty the Queen in Right of Canada 2012. Reproduced with the permission of the Minister of Agriculture.<\/jats:p>","DOI":"10.1002\/dta.1355","type":"journal-article","created":{"date-parts":[[2012,7,31]],"date-time":"2012-07-31T14:40:35Z","timestamp":1343745635000},"page":"103-111","source":"Crossref","is-referenced-by-count":62,"title":["The challenges of developing a generic extraction procedure to analyze multi\u2010class veterinary drug residues in milk and honey using ultra\u2010high pressure liquid chromatography quadrupole time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"4","author":[{"given":"Jian","family":"Wang","sequence":"first","affiliation":[{"name":"Canadian Food Inspection Agency Calgary Laboratory  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Leung","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency Calgary Laboratory  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,7,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2003.12.007"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2005.03.008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.02.037"},{"key":"e_1_2_7_5_1","unstructured":"FDA Center for Veterinary Medicine.FDA Approved Animal Drug Products. 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